University of Oulu

Joan C Hinojosa, Leonardo Dapporto, Ernst Brockmann, Vlad Dincă, Valentin Tikhonov, Nick Grishin, Vladimir A Lukhtanov, Roger Vila, Overlooked cryptic diversity in Muschampia (Lepidoptera: Hesperiidae) adds two species to the European butterfly fauna, Zoological Journal of the Linnean Society, Volume 193, Issue 3, November 2021, Pages 847–859, https://doi.org/10.1093/zoolinnean/zlaa171

Overlooked cryptic diversity in Muschampia (Lepidoptera: Hesperiidae) adds two species to the European butterfly fauna

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Author: Hinojosa, Joan C.1; Dapporto, Leonardo2; Brockmann, Ernst3;
Organizations: 1Institut de Biologia Evolutive (CSIC-UPF), Passeig de la Barceloneta 37-49, Barcelona, Spain
2ZEN lab, Dipartimento di Biologia, University of Florence, via Madonna del Piano 6, 50019 Sesto Fiorentino, Italy
3Laubacher Straße 4, 35423 Lich, Hessen, Germany
4Ecology and Genetics Research Unit, University of Oulu, Finland
5North Caucasus Federal University, Ulitsa Pushkina 1, Stavropol, Russia
6Howard Hughes Medical Institute, 4000 Jones Bridge Road, Chevy Chase, MD 20815, USA
7Department of Biophysics, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX, USA
8Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX, USA
9Department of Karyosystematics, Zoological Institute of the Russian Academy of Sciences, Universitetskaya Naberezhnaya 1, St. Petersburg, Russia
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 1.7 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2022030922698
Language: English
Published: Oxford University Press, 2021
Publish Date: 2022-03-09
Description:

Abstract

Cryptic species represent a challenge for documenting global biodiversity. Even in well-studied groups, such as European butterflies, the application of integrative approaches has allowed the recognition of an unexpected number of cryptic taxa. Here, we combine the analysis of mitochondrial (cytochrome c oxidase I, COI) and nuclear (internal transcribed spacer 2, ITS2) markers with geometric morphometrics of the male genitalia to study diversity within the butterfly Muschampia proto. The nuclear marker reveals three well-supported and deeply diverged lineages, which are also detected based on mitochondrial DNA, although the latter recovers one of them as paraphyletic with poor support. These lineages also present distinct male genital characters, which allow blind assignment of > 97% of specimens when applying a jackknife procedure. We conclude that M. proto comprises three cryptic species that started to differentiate ~2 Mya: M. proto, distributed in northern Africa, the Iberian Peninsula and southern France; Muschampia alta comb. & stat. nov., occurring in southern Italy and the Balkan Peninsula; and Muschampia proteides, present in the easternmost part of Europe, the Near East and Iran. This discovery adds two new species to the European butterfly fauna and highlights the necessity to continue investigating potential cryptic diversity.

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Series: Zoological journal of the Linnean Society
ISSN: 0024-4082
ISSN-E: 1096-3642
ISSN-L: 0024-4082
Volume: 193
Issue: 3
Pages: 847 - 859
DOI: 10.1093/zoolinnean/zlaa171
OADOI: https://oadoi.org/10.1093/zoolinnean/zlaa171
Type of Publication: A1 Journal article – refereed
Field of Science: 1181 Ecology, evolutionary biology
Subjects:
Funding: We are grateful to all the colleagues who provided samples used in this study. Financial support for this research was provided by projects PID2019-107078GB-I00 / Agencia Estatal de Investigación / 10.13039/501100011033 and 2017-SGR-991 (Generalitat de Catalunya) to R.V., by a predoctoral fellowship BES-2017-080641 (Ministerio de Economía y Empresa de España) to J.C.H., by the Academy of Finland (decision no. 328895) to V.D. and by project RSF 19-14-00202 (from the Russian Science Foundation to the Zoological Institute of the Russian Academy of Sciences) to V.A.L., and by the project Servizio di attuazione delle azioni per la protezione degli impollinatori e diffusione dell’entomofauna del Parco Nazionale dell’Alta Murgia to L.D.
Copyright information: © 2021 The Linnean Society of London, Zoological Journal of the Linnean Society, 2021, 193, 847–859. This is the peer reviewed version of the following article: Joan C Hinojosa, Leonardo Dapporto, Ernst Brockmann, Vlad Dincă, Valentin Tikhonov, Nick Grishin, Vladimir A Lukhtanov, Roger Vila, Overlooked cryptic diversity in Muschampia (Lepidoptera: Hesperiidae) adds two species to the European butterfly fauna, Zoological Journal of the Linnean Society, Volume 193, Issue 3, November 2021, Pages 847–859, which has been published in final form at https://doi.org/10.1093/zoolinnean/zlaa171. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited