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Juha Salokannel, Kyung Min Lee, Aki Rinne, Marko Mutanen, ddRAD Sequencing Sheds Light on Low Interspecific and High Intraspecific mtDNA Divergences in Two Groups of Caddisflies, Insect Systematics and Diversity, Volume 5, Issue 5, September 2021, 4, https://doi.org/10.1093/isd/ixab013

ddRAD sequencing sheds light on low interspecific and high intraspecific mtDNA divergences in two groups of caddisflies

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Author: Salokannel, Juha1; Lee, Kyung Min2,3; Rinne, Aki4;
Organizations: 1Siikinkatu 13, 33710 Tampere, Finland
2Ecology and Genetics Research Unit, PO Box 3000, FI-90014, University of Oulu, Finland
3Present address: Zoology Unit, Finnish Museum of Natural History, University of Helsinki, Finland
4Merikasarminkatu 8 D 53, 00160 Helsinki, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 13.2 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe2022032424888
Language: English
Published: Oxford University Press, 2021
Publish Date: 2022-03-24
Description:

Abstract

Large-scale global efforts on DNA barcoding have repeatedly revealed unexpected patterns of variability in mtDNA, including deep intraspecific divergences and haplotype sharing between species. Understanding the evolutionary causes behind these patterns calls for insights from the nuclear genome. While building a near-complete DNA barcode library of Finnish caddisflies, a case of barcode-sharing and some cases of deep intraspecific divergences were observed. In this study, the Apatania zonella (Zetterstedt, 1840) group and three Limnephilus Leach, 1815 species were studied using double digest RAD sequencing (ddRAD-seq), morphology, and DNA barcoding. The results support the present species boundaries in the A. zonella group species. A morphologically distinct but mitogenetically nondistinct taxon related to parthenogenetic Apatania hispida (Forsslund, 1930) got only weak support for its validity as a distinct species. The morphology and genomic-scale data do not indicate cryptic diversity in any of the three Limnephilus species despite the observed deep intraspecific divergences in DNA barcodes. This demonstrates that polymorphism in mtDNA may not reflect cryptic diversity, but mitonuclear discordance due to other evolutionary causes.

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Series: Insect systematics and diversity
ISSN: 2399-3421
ISSN-E: 2399-3421
ISSN-L: 2399-3421
Volume: 5
Issue: 5
Article number: 4
DOI: 10.1093/isd/ixab013
OADOI: https://oadoi.org/10.1093/isd/ixab013
Type of Publication: A1 Journal article – refereed
Field of Science: 1181 Ecology, evolutionary biology
Subjects:
Apatanidae
DNA barcoding
Limnephilidae
Trichoptera
ddRAD sequencing
Article
Funding: This work was supported by the Academy of Finland (grant number 277984) to M.M. and the Kvantum Institute of the Oulu University to K.M.L. Barcoding was supported by the Academy of Finland, the Kone Foundation, and the Finnish Cultural Foundation through grants to the Finnish Barcode of Life (FinBOL) initiative and the Finnish Biodiversity Information Facility (FinBIF).
Academy of Finland Grant Number: 277984
Detailed Information: 277984 (Academy of Finland Funding decision)
Copyright information: © The Author(s) 2021. Published by Oxford University Press on behalf of Entomological Society of America. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
  https://creativecommons.org/licenses/by/4.0/