University of Oulu

Marzia Iarossi, Aliaksandr Hubarevich, Giuseppina Iachetta, Michele Dipalo, Jian-An Huang, Daniel Darvill, Francesco De Angelis, Probing ND7/23 neuronal cells before and after differentiation with SERS using Sharp-tipped Au nanopyramid arrays, Sensors and Actuators B: Chemical, Volume 361, 2022, 131724, ISSN 0925-4005,

Probing ND7/23 neuronal cells before and after differentiation with SERS using Sharp-tipped Au nanopyramid arrays

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Author: Iarossi, Marzia1; Hubarevich, Aliaksandr1; Iachetta, Giuseppina1;
Organizations: 1Istituto Italiano di Tecnologia, Via Morego 30, Genova 16136, Italy
2Faculty of Medicine, Faculty of Biochemistry and Molecular Medicine, University of Oulu, 2125B, Aapistie 5°, Oulu 90220, Finland
3Department of Materials, Prince Consort Rd, South Kensington, Imperial College, London SW7, UK
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 5.2 MB)
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Language: English
Published: Elsevier, 2022
Publish Date: 2022-04-26


The development of local plasmonic nano sensors which are sensitive, whilst remaining non-invasive with high cell viability is challenging but of great interest for the investigation of cellular processes. With this aim, we developed an effective SERS active biointerface to monitor cell activity and discriminate between undifferentiated and differentiated neurons through principal component analysis. We propose a plasmonic tipped Au nanopyramids (Au NPs) array, which benefits from high aspect ratios and sharp tips that are excellent SERS sensors. To realise the structure, we developed a large-scale inexpensive fabrication route based on the assembly of charged nanospheres used as a mask by means of a colloidal lithography technique, to better space and shape the NPs. Here we show that Au NPs with tip curvatures of 10 nm and localized plasmon resonance at 785 nm can non-destructively probe ND7/23 neurons. We prove that these tips allow us to track ND7/23 neurons on the SERS substrate, detecting both the membrane constituents, proteins and lipids, and even intracellular DNA/RNA fragments.

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Series: Sensors and actuators. B, Chemical
ISSN: 0925-4005
ISSN-E: 1873-3077
ISSN-L: 0925-4005
Volume: 361
Article number: 131724
DOI: 10.1016/j.snb.2022.131724
Type of Publication: A1 Journal article – refereed
Field of Science: 221 Nanotechnology
Funding: The authors acknowledge the financial support of Istituto Italiano di Tecnologia. This work was partially supported by the European Union’s H2020 Research and Innovation Programme “TOX-Free”, grant agreement number 964518.
Copyright information: © 2022 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (