Human transcription factor protein interaction networks
|Author:||Göös, Helka1,2; Kinnunen, Matias1; Salokas, Kari1;|
1Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, Finland
2Present address: Finnish Red Cross Blood Service, Helsinki, Finland
3Biocenter Oulu and Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland
4Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University Shanghai Cancer Center, Shanghai Medical College of Fudan University, Shanghai, China
|Online Access:||PDF Full Text (PDF, 1.8 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe2022051335109
|Publish Date:|| 2022-08-22
Transcription factors (TFs) interact with several other proteins in the process of transcriptional regulation. Here, we identify 6703 and 1536 protein–protein interactions for 109 different human TFs through proximity-dependent biotinylation (BioID) and affinity purification mass spectrometry (AP-MS), respectively. The BioID analysis identifies more high-confidence interactions, highlighting the transient and dynamic nature of many of the TF interactions. By performing clustering and correlation analyses, we identify subgroups of TFs associated with specific biological functions, such as RNA splicing or chromatin remodeling. We also observe 202 TF-TF interactions, of which 118 are interactions with nuclear factor 1 (NFI) family members, indicating uncharacterized cross-talk between NFI signaling and other TF signaling pathways. Moreover, TF interactions with basal transcription machinery are mainly observed through TFIID and SAGA complexes. This study provides a rich resource of human TF interactions and also act as a starting point for future studies aimed at understanding TF-mediated transcription.
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
1182 Biochemistry, cell and molecular biology
We would like to thank the Orion Research Foundation sr, Finnish Cultural Foundation (HG), Academy of Finland (288475 and 294173), Sigrid Jusélius Foundation, Biocentrum Helsinki, University of Helsinki Three-year Research Grant, Biocentrum Finland, HiLIFE, Instrumentarium Research Foundation (MV), Jane and Aatos Erkko Foundation and Finnish Cancer Foundation (GW).
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