University of Oulu

Zhu, R., Avsievich, T., Su, X., Bykov, A., Popov, A., & Meglinski, I. (2022). Hemorheological alterations of red blood cells induced by 450-nm and 520-nm laser radiation. Journal of Photochemistry and Photobiology B: Biology, 230, 112438.

Hemorheological alterations of red blood cells induced by 450-nm and 520-nm laser radiation

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Author: Zhu, Ruixue1; Avsievich, Tatiana1; Su, Xinyang2;
Organizations: 1Optoelectronics and Measurement Techniques, University of Oulu, 90570 Oulu, Finland
2School of Science, Beijing Jiaotong University, 100044 Beijing, China
3VTT Technical Research Centre of Finland, 90590 Oulu, Finland
4Interdisciplinary Laboratory of Biophotonics, National Research Tomsk State University, 634050 Tomsk, Russia
5Institute of Clinical Medicine N.V. Sklifosovsky, I.M. Sechenov First Moscow State Medical University, Moscow 129090, Russia
6REC Fundamental and Applied Photonics, Nanophotonics, Immanuel Kant Baltic Federal University, Kaliningrad 236016, Russia
7College of Engineering and Physical Sciences, Aston University, Birmingham B4 7ET, UK
8V.A. Negovsky Scientific Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow 107031, Russia
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 4.3 MB)
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Language: English
Published: Elsevier, 2022
Publish Date: 2022-07-01


Proper rheological properties of red blood cells (RBC) including flexibility and aggregability are essential for healthy blood microcirculation. Excessive RBC aggregation has been observed to be associated with many pathological conditions and is crucial in acute circulatory problems. Low-level laser radiation (LLLR) has been found to have positive effects on the rheology of human blood, however, the detailed mechanisms of blood photobiomodulation remains unclear. In this study, utilizing the single-cell technique optical tweezers (OT) and traditional light microscopy, the influence of photobiomodulation of human RBC was examined under different conditions of laser irradiation. The results revealed that high radiant exposure (over 170.5 J/cm² radiant fluence) caused enhanced RBC aggregation and cell shape transformation while the aggregation force between single RBC remained unchanged. LLLR with radiant fluence below 9.5 J/cm² by 450 nm wavelength improved the RBC deformability, weakened the strength of cell-cell interaction in the RBC disaggregation process, and showed rejuvenating effects on RBC suspended in a harsh cell environment.

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Series: Journal of photochemistry and photobiology. B, Biology
ISSN: 1011-1344
ISSN-E: 1873-2682
ISSN-L: 1011-1344
Volume: 230
Article number: 112438
DOI: 10.1016/j.jphotobiol.2022.112438
Type of Publication: A1 Journal article – refereed
Field of Science: 213 Electronic, automation and communications engineering, electronics
Funding: This work was funded by China Scholarship Council (CSC No. 201706410089, R.Z.) and the Suomen Kulttuurirahasto (grant No. 00190188, T.A.). The authors also acknowledge the contribution of Russian Science Foundation (project: 19-72-30012) and support from the Academy of Finland (project 325097). I.M. acknowledges the support from the Leverhulme Trust and the Royal Society (Ref. no.: APX111232 APEX Awards 2021). The research was carried out with the support of a grant under the Decree of the Government of the Russian Federation No. 220 of 09 April 2010 (Agreement No. 075-15-2021-615 of 04 June 2021).
Academy of Finland Grant Number: 325097
Detailed Information: 325097 (Academy of Finland Funding decision)
Copyright information: © 2022 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (