Herpesvirus infections in adenoids in patients with chronic adenotonsillar disease
|Author:||Ivaska, Lotta E.1; Silvoniemi, Antti1; Mikola, Emilia1,2;|
1Department of Otorhinolaryngology—Head and Neck Surgery, Turku University Hospital, University of Turku, Turku, Finland
2Department of Otorhinolaryngology, Satakunta Central Hospital, Pori, Finland
3Institute of Biomedicine, Division of Infections and Immunity, University of Turku, Turku, Finland
4Department of Clinical Microbiology, Turku University Hospital, Turku, Finland
5Department of Pediatrics and Adolescent Medicine, Turku University Hospital, University of Turku, Turku, Finland
6PEDEGO Research Unit, Medical Research Center, University of Oulu, Oulu, Finland
7Department of Pediatrics and Adolescent Medicine, Oulu University Hospital, Oulu, Finland
|Online Access:||PDF Full Text (PDF, 0.8 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe2022090957961
John Wiley & Sons,
|Publish Date:|| 2022-09-09
Adenoids and tonsils have gained interest as a new in vivo model to study local immune functions and virus reservoirs. Especially herpesviruses are interesting because their prevalence and persistence in local lymphoid tissue are incompletely known. Our aim was to study herpesvirus and common respiratory virus infections in nonacutely ill adenotonsillar surgery patients. Adenoid and/or palatine tonsil tissue and nasopharyngeal aspirate (NPA) samples were collected from elective adenoidectomy (n = 45) and adenotonsillectomy (n = 44) patients (median age: 5, range: 1–20). Real-time polymerase chain reaction was used to detect 22 distinct viruses from collected samples. The overall prevalence of herpesviruses was 89% and respiratory viruses 94%. Human herpesviruses 6 (HHV6), 7 (HHV7), and Epstein–Barr virus (EBV) were found, respectively, in adenoids (33%, 26%, 25%), tonsils (45%, 52%, 23%), and NPA (46%, 38%, 25%). Copy numbers of the HHV6 and HHV7 genome were significantly higher in tonsils than in adenoids. Patients with intra-adenoid HHV6 were younger than those without. Detection rates of EBV and HHV7 showed agreement between corresponding sample types. This study shows that adenoid and tonsil tissues commonly harbor human herpes- and respiratory viruses, and it shows the differences in virus findings between sample types.
Journal of medical virology
|Pages:||4470 - 4477|
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
1183 Plant biology, microbiology, virology
This study was supported by the Finnish Cultural Foundation and the Finnish Medical Foundation, Helsinki, Finland and University of Turku, Turku, Finland.
© 2022 The Authors. Journal of Medical Virology published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.