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Association between mitochondrial DNA haplogroups J and K, serum branched-chain amino acids and lowered capability for endurance exercise |
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| Author: | Kiiskilä, Jukka M.1,2; Hassinen, Ilmo E.3; Kettunen, Johannes4,5,6,7; |
| Organizations: |
1Research Unit of Clinical Neuroscience, University of Oulu, P.O. Box 5000, 90014, Oulu, Finland 2Department of Neurology and Medical Research Center, Oulu University Hospital, Oulu, Finland 3Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland
4Computational Medicine, Faculty of Medicine, University of Oulu, Oulu, Finland
5Center for Life Course Health Research, Faculty of Medicine, University of Oulu, Oulu, Finland 6Biocenter Oulu, University of Oulu, Oulu, Finland 7Finnish Institute for Health and Welfare, Helsinki, Finland 8Rovaniemi Health Center, Rovaniemi, Finland 9Unit of General Practice, Oulu University Hospital, Oulu, Finland 10Unit of Primary Health Care, Oulu University Hospital, Oulu, Finland 11Healthcare and Social Services of Selänne, Pyhäjärvi, Finland |
| Format: | article |
| Version: | published version |
| Access: | open |
| Online Access: | PDF Full Text (PDF, 1 MB) |
| Persistent link: | http://urn.fi/urn:nbn:fi-fe2022091258313 |
| Language: | English |
| Published: |
Springer Nature,
2022
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| Publish Date: | 2022-09-12 |
| Description: |
AbstractBackground: Endurance exercise training promotes the catabolism of branched-chain amino acids (BCAAs) in skeletal muscles. We have previously shown that mitochondrial DNA (mtDNA) haplogroups J and K are markers of low responders in endurance training. In this paper, we hypothesize that BCAA catabolism is a surrogate marker of lower respiratory chain activity attributed to these haplogroups. We evaluated whether exercise-induced changes in amino acid concentrations differ between subjects harbouring mtDNA haplogroups J or K and those with non-JK haplogroups. Methods: Finnish male conscripts (N = 633) undertook the 12-min Cooper running test at the beginning and end of their military service. The intervention during the service mainly included endurance aerobic exercise and sports-related muscle training. Concentrations of seven amino acids were analysed in the serum using a high-throughput ¹H NMR metabolomics platform. Total DNA was extracted from whole blood, and restriction fragment analysis was used to determine mtDNA haplogroups J and K. Results: The concentrations of the seven amino acids were higher following the intervention, with the exception of phenylalanine; interestingly, the increase in the concentrations of three BCAAs was larger in subjects with haplogroup J or K than in subjects with non-JK haplogroups (p = 0.029). MtDNA haplogroups J and K share two common nonsynonymous variants. Structural analysis based on crystallographic data on bovine complexes I and III revealed that the Leu18 variant in cytochrome b encoded by m.14798T > C may interfere with ubiquinone binding at the Qi site in complex III. Conclusions: The increase in the concentrations of serum BCAAs following exercise intervention differs between subjects harbouring mtDNA haplogroup J or K and those harbouring non-JK haplogroups. Lower response in endurance training and difference in exercise-induced increase in the concentrations of serum BCAAs suggest decreased respiratory chain activity. Haplogroups J and K share m.14798T > C in MT-CYB, which may hamper the function of complex III. see all
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| Series: |
BMC sports science, medicine and rehabilitation |
| ISSN: | 2052-1847 |
| ISSN-E: | 2052-1847 |
| ISSN-L: | 2052-1847 |
| Volume: | 14 |
| Issue: | 1 |
| Article number: | 95 |
| DOI: | 10.1186/s13102-022-00485-3 |
| OADOI: | https://oadoi.org/10.1186/s13102-022-00485-3 |
| Type of Publication: |
A1 Journal article – refereed |
| Field of Science: |
1182 Biochemistry, cell and molecular biology |
| Subjects: | |
| Funding: |
This study was funded by grants from the Sigrid Juselius Foundation (K.M., J.K.). The work was also supported through funds from the Academy of Finland (J.K.) and the Novo Nordisk Foundation (J.K.). The funding body did not have any role in the design of the study, as well as in the collection, analysis or interpretation of the data or writing of the manuscript. |
| Copyright information: |
© The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
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https://creativecommons.org/licenses/by/4.0/ |