Elomaa, H., Ahtiainen, M., Väyrynen, S.A. et al. Prognostic significance of spatial and density analysis of T lymphocytes in colorectal cancer. Br J Cancer 127, 514–523 (2022). https://doi.org/10.1038/s41416-022-01822-6
Prognostic significance of spatial and density analysis of T lymphocytes in colorectal cancer
|Author:||Elomaa, Hanna1,2; Ahtiainen, Maarit3; Väyrynen, Sara A.4;|
1Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, Finland
2Department of Education and Research, Central Finland Health Care District, Jyväskylä, Finland
3Department of Pathology, Central Finland Health Care District, Jyväskylä, Finland
4Department of Internal Medicine, Oulu University Hospital, Oulu, Finland
5Program in MPE Molecular Pathological Epidemiology, Department of Pathology, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA, USA
6Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, MA, USA
7Broad Institute of MIT and Harvard, Cambridge, MA, USA
8Cancer Immunology and Cancer Epidemiology Programs, Dana-Farber Harvard Cancer Center, Boston, MA, USA
9Surgery Research Unit, Cancer and Translational Medicine Research Unit, Medical Research Center Oulu, Oulu University Hospital, and University of Oulu, Oulu, Finland
10Department of Gastroenterology and Alimentary Tract Surgery, Tampere University Hospital, Tampere, Finland
11Department of Gastrointestinal Surgery, Helsinki University Hospital, Helsinki, Finland
12Applied Tumor Genomics Research Program, University of Helsinki, Helsinki, Finland
13Cancer and Translational Medicine Research Unit, Medical Research Center Oulu, Oulu University Hospital, and University of Oulu, Oulu, Finland
14Faculty of Sport and Health Sciences, University of Jyväskylä, Jyväskylä, Finland
|Online Access:||PDF Full Text (PDF, 1.3 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe2022102162732
|Publish Date:|| 2022-10-21
Background: Although high T cell density is a strong favourable prognostic factor in colorectal cancer, the significance of the spatial distribution of T cells is incompletely understood. We aimed to evaluate the prognostic significance of tumour cell-T cell co-localisation and T cell densities.
Methods: We analysed CD3 and CD8 immunohistochemistry in a study cohort of 983 colorectal cancer patients and a validation cohort (N = 246). Individual immune and tumour cells were identified to calculate T cell densities (to derive T cell density score) and G-cross function values, estimating the likelihood of tumour cells being co-located with T cells within 20 µm radius (to derive T cell proximity score).
Results: High T cell proximity score associated with longer cancer-specific survival in both the study cohort [adjusted HR for high (vs. low) 0.33, 95% CI 0.20–0.52, Ptrend < 0.0001] and the validation cohort [adjusted HR for high (vs. low) 0.15, 95% CI 0.05–0.45, Ptrend < 0.0001] and its prognostic value was independent of T cell density score.
Conclusions: The spatial point pattern analysis of tumour cell-T cell co-localisation could provide detailed information on colorectal cancer prognosis, supporting the value of spatial measurement of T cell infiltrates as a novel, robust tumour-immune biomarker.
British journal of cancer
|Pages:||514 - 523|
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
This study was funded by Cancer Foundation Finland (59-5619 to JPV). SO’s effort was supported in part by a U.S. National Institutes of Health grant (R35 CA197735). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. Open Access funding provided by University of Oulu including Oulu University Hospital.
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