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Bacterial extracellular vesicles in the microbiome of first-pass meconium in newborn infants |
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| Author: | Turunen, Jenni1,2; Tejesvi, Mysore V.2,3; Suokas, Marko2; |
| Organizations: |
1Research Unit of Clinical Medicine, University of Oulu, Oulu, Finland 2Biocenter Oulu, University of Oulu, Oulu, Finland 3Ecology and Genetics, Faculty of Science, University of Oulu, Oulu, Finland
4Research Unit of Translational Medicine, University of Oulu, Oulu, Finland
5Department of Pediatrics and Adolescent Medicine, Oulu University Hospital, Oulu, Finland |
| Format: | article |
| Version: | published version |
| Access: | open |
| Online Access: | PDF Full Text (PDF, 2.6 MB) |
| Persistent link: | http://urn.fi/urn:nbn:fi-fe2022102462768 |
| Language: | English |
| Published: |
Springer Nature,
2022
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| Publish Date: | 2022-10-24 |
| Description: |
AbstractBackground: Bacterial extracellular vesicles (EVs) are more likely to cross biological barriers than whole-cell bacteria. We previously observed EV-sized particles by electron microscopy in the first-pass meconium of newborn infants. We hypothesized that EVs may be of bacterial origin and represent a novel entity in the human microbiome during fetal and perinatal periods. Methods: We extracted EVs from first-pass meconium samples of 17 newborn infants and performed bacterial 16S rRNA gene sequencing of the vesicles. We compared the EV content from the meconium samples of infants based on the delivery mode, and in vaginal delivery samples, based on the usage of intrapartum antibiotics. Results: We found bacterial EVs in all first-pass meconium samples. All EV samples had bacterial RNA. Most of the phyla present in the samples were Firmicutes (62%), Actinobacteriota (18%), Proteobacteria (10%), and Bacteroidota (7.3%). The most abundant genera were Streptococcus (21%) and Staphylococcus (17%). The differences between the delivery mode and exposure to antibiotics were not statistically significant. Conclusions: Bacterial EVs were present in the first-pass meconium of newborn infants. Bacterial EVs may represent an important novel feature of the gut microbiome during fetal and perinatal periods. see all
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| Series: |
Pediatric research |
| ISSN: | 0031-3998 |
| ISSN-E: | 1530-0447 |
| ISSN-L: | 0031-3998 |
| Volume: | 93 |
| Issue: | 4 |
| Pages: | 887 - 896 |
| DOI: | 10.1038/s41390-022-02242-1 |
| OADOI: | https://oadoi.org/10.1038/s41390-022-02242-1 |
| Type of Publication: |
A1 Journal article – refereed |
| Field of Science: |
1183 Plant biology, microbiology, virology 3123 Gynaecology and paediatrics |
| Subjects: | |
| Funding: |
T.T. thanks the Academy of Finland for Clinical Research grant 2018–2022 and Pediatric Research Foundation grant 2019–2022. J.R. thanks the Academy of Finland for Grants 299749 and 328768. Open Access funding provided by University of Oulu including Oulu University Hospital. |
| Academy of Finland Grant Number: |
299749 328768 |
| Detailed Information: |
299749 (Academy of Finland Funding decision) 328768 (Academy of Finland Funding decision) |
| Copyright information: |
© The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
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https://creativecommons.org/licenses/by/4.0/ |