Y. Kharbanda, M. Urbańczyk, V. V. Zhivonitko, S. Mailhiot, M. I. Kettunen, V.-V. Telkki, Angew. Chem. Int. Ed. 2022, 61, e202203957; Angew. Chem. 2022, 134, e202203957.
Sensitive, efficient and portable analysis of molecular exchange processes by hyperpolarized ultrafast NMR
|Author:||Kharbanda, Yashu1; Urbańczyk, Mateusz2; Zhivonitko, Vladimir V.1;|
1NMR Research Unit, University of Oulu, Oulu, 90540 Finland
2Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland
3Kuopio Biomedical Imaging Unit, A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland
|Online Access:||PDF Full Text (PDF, 0.5 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe2022102462812
John Wiley & Sons,
|Publish Date:|| 2022-10-24
Molecular exchange processes are ubiquitous in nature. Here, we introduce a method to analyze exchange processes by using low-cost, portable, single-sided NMR instruments. The inherent magnetic field inhomogeneity of the single-sided instruments is exploited to achieve diffusion contrast of exchange sites and spatial encoding of 2D data. This so-called ultrafast diffusion exchange spectroscopy method shortens the experiment time by two to four orders of magnitude. Furthermore, because full 2D data are measured in a single scan (in a fraction of a second), the sensitivity of the experiment can be improved by several orders of magnitude using so-called nuclear spin hyperpolarization methods (in this case, dissolution dynamic nuclear polarization). As the first demonstration of the feasibility of the method in various applications, we show that the method enables quantification of intra- and extracellular exchange of water in a yeast cell suspension.
Angewandte Chemie. International edition in English
|Type of Publication:||
B1 Journal article
|Field of Science:||
114 Physical sciences
116 Chemical sciences
Financial support from the European Research Council (Project number 772110), Academy of Finland (grant nos. 323480, 340099, 332006 and 896824), Marie Skłodowska-Curie Actions (grant no. 321701), National Science Centre, Poland (grant no. 2021/41/B/ST4/01286), and the University of Oulu (Kvantum Institute) is gratefully acknowledged. Part of the work was carried out with the support of the Centre for Material Analysis, University of Oulu, Finland.
|EU Grant Number:||
(772110) UFLNMR - Ultrafast Laplace NMR
(896824) NMRCement - Zero-CO2 cement concept evaluated with novel Nuclear Magnetic Resonance (NMR)
|Academy of Finland Grant Number:||
323480 (Academy of Finland Funding decision)
340099 (Academy of Finland Funding decision)
321701 (Academy of Finland Funding decision)
The Supporting Information includes description of experimental details and UF DEXSY data simulations. The datasets and analysis scripts used in this article are available in zenodo repository at: https://doi.org/10.5281/zenodo.5560815.
© 2022 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.