University of Oulu

Istomin, N., Härma, M.-A., Akhi, R., Nissinen, A.E., Savolainen, M.J., Adeshara, K., Lehto, M., Groop, P.-H., Koivukangas, V., Hukkanen, J. and Hörkkö, S. (2022), Total fecal IgA levels increase and natural IgM antibodies decrease after gastric bypass surgery. APMIS, 130: 637-646.

Total fecal IgA levels increase and natural IgM antibodies decrease after gastric bypass surgery

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Author: Istomin, Natalie1,2,3; Härma, Mari-Anne4,5,6; Akhi, Ramin1,2;
Organizations: 1Medical Microbiology and Immunology, Research Unit of Biomedicine, University of Oulu, Oulu, Finland
2Medical Research Center Oulu, Oulu University Hospital and University of Oulu, Oulu, Finland
3Nordlab, Oulu University Hospital, Oulu, Finland
4Folkhälsan Institute of Genetics, Folkhälsan Research Center, Helsinki, Finland
5Abdominal Center, Nephrology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland
6Clinical and Molecular Metabolism, Faculty of Medicine Research Programs, University of Helsinki, Helsinki, Finland
7Research Unit of Internal Medicine and Biocenter Oulu, University of Oulu, Oulu, Finland
8Department of Surgery, Oulu University Hospital, Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 0.3 MB)
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Language: English
Published: John Wiley & Sons, 2022
Publish Date: 2022-11-25


Obesity is associated with low-grade inflammation and increased systemic oxidative stress. Roux-en-Y gastric bypass (RYGB) surgery is known to ameliorate the obesity-induced metabolic dysfunctions. We aimed to study the levels of natural antibodies in feces, before and 6 months after RYGB surgery in obese individuals with and without type 2 diabetes (T2D). Sixteen individuals with T2D and 14 non-diabetic (ND) individuals were operated. Total IgA, IgG and IgM antibody levels and specific antibodies to oxidized low-density lipoprotein (oxLDL), malondialdehyde-acetaldehyde adducts (MAA adducts), Porphyromonas gingivalis gingipain A hemagglutinin domain (Rgp44) and phosphocholine (PCho) were measured using chemiluminescence immunoassay. Total fecal IgA was elevated, while total IgM and IgG were not affected by the surgery. Fecal natural IgM specific to oxLDL decreased significantly in both T2D and ND individuals, while fecal IgM to Rgp44 and PCho decreased significantly in T2D individuals. A decrease in IgG to MAA-LDL, Rgp44 and PCho was detected. RYGB surgery increases the levels of total fecal IgA and decreases fecal natural IgG and IgM antibodies specific to oxLDL. Natural antibodies and IgA are important in maintaining the normal gut homeostasis and first-line defense against microbes, and their production is markedly altered with RYGB surgery.

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Series: APMIS
ISSN: 0903-4641
ISSN-E: 1600-0463
ISSN-L: 0903-4641
Volume: 130
Issue: 11
Pages: 637 - 646
DOI: 10.1111/apm.13268
Type of Publication: A1 Journal article – refereed
Field of Science: 3111 Biomedicine
Funding: Yrjö Jahnsson Foundation (20197174), Academy of Finland (275614, 316664, and 315568), Novo Nordisk Foundation (#NNF OC0013659), Signe and Ane Gyllenberg Foundation, Folkhälsan Research Foundation, Helsinki University Central Hospital Research Funds, the Diabetes Research Foundation, the Päivikki and Sakari Sohlberg Foundation, the Finnish Foundation for Cardiovascular Research, the Northern Finland Health Care Support Foundation, the Finnish Medical Foundation, and Wilhelm and Else Stockmann Foundation supported this study.
Copyright information: © 2022 The Authors. APMIS published by John Wiley & Sons Ltd on behalf of Scandinavian Societies for Pathology, Medical Microbiology and Immunology. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.