University of Oulu

Derkus, B., Isik, M., Eylem, C. C., Ergin, I., Camci, C. B., Bilgin, S., Elbuken, C., Arslan, Y. E., Akkulak, M., Adali, O., Kiran, F., Okesola, B. O., Nemutlu, E., Emregul, E., Xenogenic Neural Stem Cell-Derived Extracellular Nanovesicles Modulate Human Mesenchymal Stem Cell Fate and Reconstruct Metabolomic Structure. Adv. Biology 2022, 6, 2101317. https://doi.org/10.1002/adbi.202101317

Xenogenic neural stem cell-derived extracellular nanovesicles modulate human mesenchymal stem cell fate and reconstruct metabolomic structure

Saved in:
Author: Derkus, Burak1,2; Isik, Melis2; Eylem, Cemil Can3;
Organizations: 1Stem Cell Research Lab, Department of ChemistryFaculty of Science, Ankara University, Ankara, 06560 Turkey
2Interdisciplinary Research Unit for Advanced Materials (INTRAM) Department of Chemistry, Faculty of Science, Ankara University, Ankara, 06560 Turkey
3Analytical Chemistry Division, Faculty of Pharmacy, Hacettepe University, Ankara, 06530 Turkey
4Department of Surgery, Faculty of Veterinary Medicine, Ankara University, Turkey
5UNAM-National Nanotechnology Research Center, Institute of Materials Science and Nanotechnology, Bilkent University, Ankara, 06800 Turkey
6Faculty of Biochemistry and Molecular Medicine, Faculty of Medicine, University of Oulu, Oulu, 90014 Finland
7Regenerative Biomaterials Laboratory, Department of Bioengineering, Engineering Faculty, Canakkale Onsekiz Mart University, Canakkale, 17100 Turkey
8Department of Biological Sciences, Faculty of Science, Middle East Technical University, Ankara, 06800 Turkey
9Department of Biology, Faculty of Science, Ankara University, Ankara, 06560 Turkey
10Department of Eye and Vision Science, Institute of Life Course and Medical Sciences, Faculty of Medicine, University of Liverpool, Liverpool, L7 8TX UK
11Bioanalytic and Omics Laboratory, Faculty of Pharmacy, Hacettepe University, Ankara, 06530 Turkey
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 1.4 MB)
Persistent link: http://urn.fi/urn:nbn:fi-fe202301193637
Language: English
Published: John Wiley & Sons, 2022
Publish Date: 2023-01-19
Description:

Abstract

Extracellular nanovesicles, particularly exosomes, can deliver their diverse bioactive biomolecular content, including miRNAs, proteins, and lipids, thus providing a context for investigating the capability of exosomes to induce stem cells toward lineage-specific cells and tissue regeneration. In this study, it is demonstrated that rat subventricular zone neural stem cell-derived exosomes (rSVZ-NSCExo) can control neural-lineage specification of human mesenchymal stem cells (hMSCs). Microarray analysis shows that the miRNA content of rSVZ-NSCExo is a faithful representation of rSVZ tissue. Through immunocytochemistry, gene expression, and multi-omics analyses, the capability to use rSVZ-NSCExo to induce hMSCs into a neuroglial or neural stem cell phenotype and genotype in a temporal and dose-dependent manner via multiple signaling pathways is demonstrated. The current study presents a new and innovative strategy to modulate hMSCs fate by harnessing the molecular content of exosomes, thus suggesting future opportunities for rSVZ-NSCExo in nerve tissue regeneration.

see all

Series: Advanced biology
ISSN: 2701-0198
ISSN-E: 2701-0198
ISSN-L: 2701-0198
Volume: 6
Issue: 6
Article number: 2101317
DOI: 10.1002/adbi.202101317
OADOI: https://oadoi.org/10.1002/adbi.202101317
Type of Publication: A1 Journal article – refereed
Field of Science: 1182 Biochemistry, cell and molecular biology
Subjects:
Funding: This work was financially supported by the Scientific and Technological Research Council of Turkey (TUBITAK) with the grant number 116S476.
Copyright information: © 2022 Wiley-VCH GmbH. This is the peer reviewed version of the following article: Derkus, B., Isik, M., Eylem, C. C., Ergin, I., Camci, C. B., Bilgin, S., Elbuken, C., Arslan, Y. E., Akkulak, M., Adali, O., Kiran, F., Okesola, B. O., Nemutlu, E., Emregul, E., Xenogenic Neural Stem Cell-Derived Extracellular Nanovesicles Modulate Human Mesenchymal Stem Cell Fate and Reconstruct Metabolomic Structure. Adv. Biology 2022, 6, 2101317, which has been published in final form at https://doi.org/10.1002/adbi.202101317. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.