University of Oulu

Koivisto, M., Tolvanen, T.A., Toimela, T. et al. Functional human cell-based vascularised cardiac tissue model for biomedical research and testing. Sci Rep 12, 13459 (2022).

Functional human cell-based vascularised cardiac tissue model for biomedical research and testing

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Author: Koivisto, Maria1; Tolvanen, Tuomas A.1; Toimela, Tarja1;
Organizations: 1Faculty of Medicine and Health Technology, Tampere University, Arvo Ylpön katu 34, 33520, Tampere, Finland
2Biocenter Oulu Electron Microscopy Core Facility, University of Oulu, Oulu, Finland
3Computational Biology, Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland
4Oulu Centre for Cell-Matrix Research, Faculty of Biochemistry and Molecular Medicine, Biocenter Oulu, University of Oulu, Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 3.5 MB)
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Language: English
Published: Springer Nature, 2022
Publish Date: 2023-02-07


Cardiomyocytes derived from human induced pluripotent stem cells (hiPSC) are widely used in in vitro biomedical research and testing. However, fully matured, adult cardiomyocyte characteristics have not been achieved. To improve the maturity and physiological relevance of hiPSC-derived cardiomyocytes, we co-cultured them with preconstructed vascular-like networks to form a functional, human cell-based cardiac tissue model. The morphology and gene expression profiles indicated advanced maturation in the cardiac tissue model compared to those of a cardiomyocyte monoculture. The cardiac tissue model’s functionality was confirmed by measuring the effects of 32 compounds with multielectrode array and comparing results to human data. Our model predicted the cardiac effects with a predictive accuracy of 91%, sensitivity of 90% and specificity of 100%. The correlation between the effective concentration (EC50) and the reported clinical plasma concentrations was 0.952 (R2 = 0.905). The developed advanced human cell-based cardiac tissue model showed characteristics and functionality of human cardiac tissue enabling accurate transferability of gained in vitro data to human settings. The model is standardized and thus, it would be highly useful in biomedical research and cardiotoxicity testing.

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Series: Scientific reports
ISSN: 2045-2322
ISSN-E: 2045-2322
ISSN-L: 2045-2322
Volume: 12
Issue: 1
Article number: 13459
DOI: 10.1038/s41598-022-17498-0
Type of Publication: A1 Journal article – refereed
Field of Science: 3111 Biomedicine
Funding: This work was supported by Business Finland [grant number 6785/31/2017]; Academy of Finland [grant number 310527]; Pirkanmaa Cultural Foundation [grant number 50211545]; Ministry of Agriculture and Forestry [grant number 1149/]; Juliana von Wendt foundation; and Ministry of Education and Culture. The authors acknowledge the Biocenter Finland (BF), Tampere Imaging Facility (TIF) and Biocenter Oulu (BCO) Electron Microscopy Core Facilities for research infrastructure services.
Dataset Reference: A gene count matrix with corresponding metadata has been included in supplementary data.
Copyright information: © The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit