University of Oulu

Li, H., Zhang, H., Luo, W., Yuan, R., Zhao, Y., Huang, J.-A., & Yang, X. (2022). Microcontact printing of gold nanoparticle at three-phase interface as flexible substrate for SERS detection of MicroRNA. Analytica Chimica Acta, 1229, 340380.

Microcontact printing of gold nanoparticle at three-phase interface as flexible substrate for SERS detection of MicroRNA

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Author: Li, Hongying1; Zhang, Haina1; Luo, Wei1;
Organizations: 1Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education; College of Chemistry and Chemical Engineering, Southwest University, Chongqing, PR China
2Faculty of Medicine, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Aapistie 5A, 90220, Oulu, Finland
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 0.9 MB)
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Language: English
Published: Elsevier, 2022
Publish Date: 2023-09-12


The rigidity of traditional solid-state surface-enhanced Raman spectroscopy (SERS) substrate hampers their application in the curved structure for nonplanar surface test and in-situ detection. Traditionally, the flexible Raman substrates are often prepared by transferring printing of patterned nanoparticles on the flexible materials such as polymer, paper, etc. However, the replicate patterns are often produced by high-cost instruments. In this study, a low-cost and flexible SERS substrate is prepared by using a microcontact printing technology to transfer three-phase-assembled nanoparticles on a polydimethylsiloxane film, which can stabilize the assembled nanoparticles. Combining with the endonuclease Nt.BbvCI assisted amplification method, a SERS biosensor is constructed for microRNA 21 (miRNA 21) assay. This platform presents a wide dynamic range (100 fM ∼1 nM), achieving a fabulous sensitivity with limit of detection of 11.96 fM for miRNA 21. Furthermore, after being bent 90° for 50 times, the Raman intensity of the flexible substrate shows a negligible change. This versatile flexible substrate exhibits considerable potential for SERS analysis, which also opens a new avenue for preparing flexible devices.

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Series: Analytica chimica acta
ISSN: 0003-2670
ISSN-E: 1873-4324
ISSN-L: 0003-2670
Volume: 1229
Article number: 340380
DOI: 10.1016/j.aca.2022.340380
Type of Publication: A1 Journal article – refereed
Field of Science: 114 Physical sciences
Funding: This work is supported by the National Natural Science Foundation of China (22174114). Jian-An Huang acknowledges the DigiHealth-project, a strategic profiling project at the University of Oulu that is supported by the Academy of Finland (project number 326291) and the University of Oulu.
Copyright information: © 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license