University of Oulu

Jaafar, A., Holomb, R., Sdobnov, A. Y., Ocskay, Z., Jakus, Z., Tuchin, V. V., Veres, M., J. Biophotonics 2022, 15( 4), e202100332.

Ex vivo confocal Raman microspectroscopy of porcine dura mater supported by optical clearing

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Author: Jaafar, Ali1,2,3; Holomb, Roman1,4; Sdobnov, Anton Y.5,6;
Organizations: 1Institute for Solid State Physics and Optics, Wigner Research Center for Physics, Budapest, Hungary
2Institute of Physics, University of Szeged, Szeged, Hungary
3Ministry of Higher Education and Scientific Research, Baghdad, Iraq
4Department of Information Control Systems and Technologies, Uzhhorod National University, Uzhhorod, Ukraine
5Science Medical Center, Saratov State University, Saratov, Russia
6Optoelectronics and Measurement Techniques Laboratory, University of Oulu, Oulu, Finland
7Department of Physiology, Semmelweis University School of Medicine, Budapest, Hungary
8Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precision Mechanics and Control of the Russian Academy of Sciences, Saratov, Russia
9А.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia
Format: article
Version: accepted version
Access: open
Online Access: PDF Full Text (PDF, 0.7 MB)
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Language: English
Published: John Wiley & Sons, 2022
Publish Date: 2023-04-05


The effect of tissue optical clearing (TOC) to increase the probing depth and observe in-depth structure of the ex vivo porcine dura mater was studied by confocal Raman microspectroscopy (CRM). Raman intensities were significantly increased at the depth of 250 μm for all collagen bands after treatment with glycerol. The influence of glycerol on collagen hydration was also investigated. The results indicate that the process of TOC can be divided into three main steps. The first one is a fast process of tissue dehydration accompanied by collagen shrinkage while the second relatively slow process is related to the glycerol penetration into the interfibrillar space of collagen combined with swelling of tissue. The third step is collagen dissociation caused by the high concentration of glycerol. To the best of our knowledge, this study is the first example to introduce the TOC technique in assisting CRM of ex vivo dura mater in-depth probing.

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Series: Journal of biophotonics
ISSN: 1864-063X
ISSN-E: 1864-0648
ISSN-L: 1864-063X
Volume: 15
Issue: 4
Article number: e202100332
DOI: 10.1002/jbio.202100332
Type of Publication: A1 Journal article – refereed
Field of Science: 114 Physical sciences
Funding: This study was done under a scholarship of the Stipendium Hungaricum Scholarship Programme. This study was supported by the VEKOP-2.3.2-16-2016-00011 and VEKOP-2.3.2-16-2016-00002 grants, which are co-financed by the European Union and European Social Fund. VVT was supported by the grant of the Government of the Russian Federation 075-15-2019-1885.
Copyright information: © 2021 Wiley-VCH GmbH. This is the peer reviewed version of the following article: Jaafar, A., Holomb, R., Sdobnov, A. Y., Ocskay, Z., Jakus, Z., Tuchin, V. V., Veres, M., J. Biophotonics 2022, 15( 4), e202100332., which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.