University of Oulu

Lautaoja, J. H., Turner, D. C., Sharples, A. P., Kivelä, R., Pekkala, S., Hulmi, J. J., & Ylä-Outinen, L. (2023). Mimicking exercise in vitro: Effects of myotube contractions and mechanical stretch on omics. American Journal of Physiology-Cell Physiology, 324(4), C886–C892.

Mimicking exercise in vitro : effects of myotube contractions and mechanical stretch on omics

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Author: Lautaoja, J. H.1,2; Turner, D. C.3; Sharples, A. P.3;
Organizations: 1Faculty of Sport and Health Sciences, NeuroMuscular Research Center, University of Jyväskylä, Jyväskylä, Finland
2Faculty of Medicine, Research Unit of Internal Medicine, University of Oulu, Oulu, Finland
3Institute for Physical Performance, Norwegian School of Sport Sciences (NIH), Oslo, Norway
Format: article
Version: accepted version
Access: embargoed
Persistent link:
Language: English
Published: American Physiological Society, 2023
Publish Date: 2024-03-29


The number of studies using skeletal muscle (SkM) cell culture models to study exercise in vitro are rapidly expanding. Progressively, more comprehensive analysis methods, such as different omics approaches including transcriptomics, proteomics, and metabolomics have been used to examine the intra- and extracellular molecular responses to exercise mimicking stimuli in cultured myotubes. Among other techniques, exercise-like electrical pulse stimulation (EL-EPS) and mechanical stretch of SkM cells are the two most commonly used methods to mimic exercise in vitro. In this mini-review, we focus on these two approaches and their effects on the omics of myotubes and/or cell culture media. Furthermore, besides traditional two-dimensional (2-D) methods, the use of three-dimensional (3-D) SkM approaches are increasing in the field of in vitro exercise mimicry. Our aim with this mini-review is to provide the reader with an up-to-date overview of the 2-D and 3-D models and the use of omics approaches to study the molecular response to exercise in vitro.

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Series: American journal of physiology. Cell physiology
ISSN: 0363-6143
ISSN-E: 1522-1563
ISSN-L: 0363-6143
Volume: 324
Issue: 4
Pages: C886 - C892
DOI: 10.1152/ajpcell.00586.2022
Type of Publication: A2 Review article in a scientific journal
Field of Science: 315 Sport and fitness sciences
1182 Biochemistry, cell and molecular biology
Funding: This work was supported by Finnish Cultural Foundation (to J.H.L.), Emil Aaltonen Foundation (to J.H.L.), and Academy of Finland Profi5 (301824) funding (Physical ACTivity and health during the human life-Span 2; PACTS2) to the University of Jyväskylä and Profi6 (336449) funding (Fibrobesity—Preventing fibrosis related to obesity) to the University of Oulu. Research Council of Norway grant was awarded to A.P.S under project code 314157 (D.C.T. and A.P.S).
Academy of Finland Grant Number: 336449
Detailed Information: 336449 (Academy of Finland Funding decision)
Copyright information: © 2023 the American Physiological Society.