University of Oulu

Piavchenko G, Soldatov V, Venediktov A, Kartashkina N, Novikova N, Gorbunova M, Boronikhina T, Yatskovskiy A, Meglinski I and Kuznetsov S (2022) A combined use of silver pretreatment and impregnation with consequent Nissl staining for cortex and striatum architectonics study. Front. Neuroanat. 16:940993. doi: 10.3389/fnana.2022.940993

A combined use of silver pretreatment and impregnation with consequent Nissl staining for cortex and striatum architectonics study

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Author: Piavchenko, Gennadii1; Soldatov, Vladislav2; Venediktov, Artem1;
Organizations: 1Department of Histology, Cytology, and Embryology, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia
2Department of Pharmacology and Clinical Pharmacology, Belgorod National Research University, Belgorod, Russia
3Laboratory of Pathophysiology, Institute of Experimental Medicine, Saint Petersburg, Russia
4Department of Histology, Cytology, and Embryology, Orel State University named after I.S. Turgenev, Orel, Russia
5Opto-Electronics and Measurement Techniques, Faculty of Information and Electrical Engineering, University of Oulu, Oulu, Finland
6College of Engineering and Applied Science, Aston University, Birmingham, United Kingdom
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 8.9 MB)
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Language: English
Published: Frontiers Media SA, 2022
Publish Date: 2023-04-21


Despite a rapid growth in the application of modern techniques for visualization studies in life sciences, the classical methods of histological examination are yet to be outdated. Herein, we introduce a new approach that involves combining silver nitrate pretreatment and impregnation with consequent Nissl (cresyl violet) staining for cortex and striatum architectonics study on the same microscopy slide. The developed approach of hybrid staining provides a high-quality visualization of cellular and subcellular structures, including impregnated neurons (about 10%), Nissl-stained neurons (all the remaining ones), and astrocytes, as well as chromatophilic substances, nucleoli, and neuropil in paraffin sections. We provide a comparative study of the neuronal architectonics in both the motor cortex and striatum based on the differences in their tinctorial properties. In addition to a comparative study of the neuronal architectonics in both the motor cortex and striatum, the traditional methods to stain the cortex (motor and piriform) and the striatum are considered. The proposed staining approach compiles the routine conventional methods for thin sections, expanding avenues for more advanced examination of neurons, blood–brain barrier components, and fibers both under normal and pathological conditions. One of the main hallmarks of our method is the ability to detect changes in the number of glial cells. The results of astrocyte visualization in the motor cortex obtained by the developed technique agree well with the alternative studies by glial fibrillary acidic protein (GFAP) immunohistochemical reaction. The presented approach of combined staining has great potential in current histological practice, in particular for the evaluation of several neurological disorders in clinical, pre-clinical, or neurobiological animal studies.

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Series: Frontiers in neuroanatomy
ISSN: 1662-5129
ISSN-E: 1662-5129
ISSN-L: 1662-5129
Volume: 16
Article number: 940993
DOI: 10.3389/fnana.2022.940993
Type of Publication: A1 Journal article – refereed
Field of Science: 213 Electronic, automation and communications engineering, electronics
Funding: This research was funded by the Ministry of Science and Higher Education of the Russian Federation within the framework of State support for the creation and development of World-Class Research Centers “Digital Biodesign and Personalized Healthcare” (grant number: 075-15-2022-304). IM also acknowledges partial support from the Academy of Finland (grant projects: 351068 and 325097).
Academy of Finland Grant Number: 351068
Detailed Information: 351068 (Academy of Finland Funding decision)
325097 (Academy of Finland Funding decision)
Dataset Reference: The original contributions presented in this study are included in the article/supplementary material, further inquiries can be directed to the corresponding authors.
Copyright information: © 2022 Piavchenko, Soldatov, Venediktov, Kartashkina, Novikova, Gorbunova, Boronikhina, Yatskovskiy, Meglinski and Kuznetsov. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).