University of Oulu

Pratiwi, F.W. et al. (2023). Biogenesis of Mesoporous Silica Nanoparticles Enclosed in Extracellular Vesicles by Mouse Renal Adenocarcinoma Cells. In: Vainio, S. (eds) Cell-Secreted Vesicles. Methods in Molecular Biology, vol 2668. Humana, New York, NY.

Biogenesis of mesoporous silica nanoparticles enclosed in extracellular vesicles by mouse renal adenocarcinoma cells

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Author: Pratiwi, Feby Wijaya1; Keerthanaa Balasubramanian Shanthi1; Makieieva, Olha1;
Organizations: 1Laboratory of Developmental Biology, Disease Networks Research Unit, Faculty of Biochemistry and Molecular Medicine, University of Oulu and Kvantum Institute, Oulu, Finland
2Department of Chemistry, National Taiwan University, Taipei, Taiwan
3Biocenter Oulu, Department of Pathology, Oulu University Hospital, University of Oulu, Oulu, Finland
4Graduate Institute of Nanomedicine and Medical Engineering, Taipei Medical University, Taipei, Taiwan
Format: article
Version: accepted version
Access: embargoed
Persistent link:
Language: English
Published: Humana Press, 2023
Publish Date: 2025-05-05


Integrating the versatility of synthetic nanoparticles to natural biomaterials, such as cells or cell membranes, has gained considerable attention as promising alternative cargo delivery platforms in recent years. Extracellular vesicles (EVs), natural nanomaterials composed of a protein-rich lipid bilayer secreted by cells, have also shown advantages and great potential as a nano delivery platform in combination with synthetic particles due to their specific natural properties in overcoming several biology hurdles possessed in the recipient cell. Therefore, the preservation of EV’s origin properties is critical for their application as nanocarriers. This chapter will describe the encapsulation procedure of MSN encapsulated in EV membrane derived from mouse renal adenocarcinoma (Renca) cells through biogenesis. The FMSN-enclosed EVs produced through this approach still contain preserved EV’s natural membrane properties.

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Series: Methods in molecular biology
ISSN: 1064-3745
ISSN-E: 1940-6029
ISSN-L: 1064-3745
ISBN: 978-1-0716-3203-1
ISBN Print: 978-1-0716-3202-4
Volume: 2668
Pages: 241 - 256
Host publication: Cell-secreted vesicles : methods and protocols
Host publication editor: Vainio, Seppo
Type of Publication: D2 Article in a professional research book (incl. editor’s introduction)
Field of Science: 1182 Biochemistry, cell and molecular biology
Copyright information: © 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.