University of Oulu

Li, Y., Wang, Y., Tian, J., & Huang, J.-A. (2023). Detection of cell-derived exosomes via surface-enhanced raman scattering using aggregated silver nanoparticles. In S. Vainio (Ed.), Cell-Secreted Vesicles (Vol. 2668, pp. 15–22). Springer US. https://doi.org/10.1007/978-1-0716-3203-1_2

Detection of cell-derived exosomes via surface-enhanced Raman scattering using aggregated silver nanoparticles

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Author: Li, Yang1; Wang, Yunpeng1; Tian, Jinwei2;
Organizations: 1College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang, China
2Department of Cardiology, The Second Affiliated Hospital of Harbin Medical University, The Key Laboratory of Myocardial Ischemia, Ministry of Education, Harbin Medical University, Harbin, Heilongjiang, China
3Faculty of Medicine, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland
Format: article
Version: accepted version
Access: embargoed
Persistent link: http://urn.fi/urn:nbn:fi-fe2023051243929
Language: English
Published: Humana Press, 2023
Publish Date: 2025-05-05
Description:

Abstract

Exosomes are small extracellular vesicles that contain RNA, lipids, and proteins and can act as cellular messengers, carrying information to cells and tissues in the body. Thus, sensitive, label-free, and multiplexed analysis of exosomes may help in early diagnosis of important diseases. Here, we describe the process of pretreatment of cell-derived exosomes, preparation of surface-enhanced Raman scattering (SERS) substrates, and label-free SERS detection of exosomes using sodium borohydride aggregators. This method can enable the observation of SERS signals of exosomes that are clear and stable and have a good signal-to-noise ratio.

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Series: Methods in molecular biology
ISSN: 1064-3745
ISSN-E: 1940-6029
ISSN-L: 1064-3745
ISBN: 978-1-0716-3203-1
ISBN Print: 978-1-0716-3202-4
Issue: 2668
Pages: 15 - 22
DOI: 10.1007/978-1-0716-3203-1_2
OADOI: https://oadoi.org/10.1007/978-1-0716-3203-1_2
Host publication: Cell-secreted vesicles : methods and protocols
Host publication editor: Vainio, Seppo
Type of Publication: D2 Article in a professional research book (incl. editor’s introduction)
Field of Science: 1182 Biochemistry, cell and molecular biology
Subjects:
Funding: HMU Marshal Initiative Funding (No: HMUMIF-21012); Jian-An Huang acknowledges the DigiHealth-project, a strategic profiling project at the University of Oulu that is supported by the Academy of Finland (project number 326291) and the University of Oulu.
Academy of Finland Grant Number: 326291
Detailed Information: 326291 (Academy of Finland Funding decision)
Copyright information: © 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. This version of the article has been accepted for publication, after peer review, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/978-1-0716-3203-1_2. Use of this Accepted Version is subject to the publisher’s Accepted Manuscript terms of use https://www.springernature.com/gp/open-research/policies/accepted-manuscript-terms”.