Optogenetic control of bacterial expression by red light
|Author:||Multamäki, Elina1; de Fuentes, Andrés García2; Sieryi, Oleksii3;|
1Department of Anatomy, University of Helsinki, Helsinki 00014, Finland
2Lehrstuhl für Biochemie, Photobiochemie, Universität Bayreuth, Bayreuth 95447, Germany
3Optoelectronics and Measurement Techniques, University of Oulu, Oulu 90014, Finland
4Lehrstuhl für Spektroskopie weicher Materie, Universität Bayreuth, Bayreuth 95447, Germany
5College of Engineering and Physical Sciences, Aston University, Birmingham B4 7ET, U.K.
6Department of Biological and Environmental Science, Nanoscience Center, University of Jyvaskyla, Jyvaskyla 40014, Finland
|Online Access:||PDF Full Text (PDF, 4.8 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe2023060953175
American Chemical Society,
|Publish Date:|| 2023-06-09
In optogenetics, as in nature, sensory photoreceptors serve to control cellular processes by light. Bacteriophytochrome (BphP) photoreceptors sense red and far-red light via a biliverdin chromophore and, in response, cycle between the spectroscopically, structurally, and functionally distinct Pr and Pfr states. BphPs commonly belong to two-component systems that control the phosphorylation of cognate response regulators and downstream gene expression through histidine kinase modules. We recently demonstrated that the paradigm BphP from Deinococcus radiodurans exclusively acts as a phosphatase but that its photosensory module can control the histidine kinase activity of homologous receptors. Here, we apply this insight to reprogram two widely used setups for bacterial gene expression from blue-light to red-light control. The resultant pREDusk and pREDawn systems allow gene expression to be regulated down and up, respectively, uniformly under red light by 100-fold or more. Both setups are realized as portable, single plasmids that encode all necessary components including the biliverdin-producing machinery. The triggering by red light affords high spatial resolution down to the single-cell level. As pREDusk and pREDawn respond sensitively to red light, they support multiplexing with optogenetic systems sensitive to other light colors. Owing to the superior tissue penetration of red light, the pREDawn system can be triggered at therapeutically safe light intensities through material layers, replicating the optical properties of the skin and skull. Given these advantages, pREDusk and pREDawn enable red-light-regulated expression for diverse use cases in bacteria.
ACS synthetic biology
|Pages:||3354 - 3367|
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
1184 Genetics, developmental biology, physiology
213 Electronic, automation and communications engineering, electronics
This work was supported by the Academy of Finland grant 330678 (H.T.), Three-year grant 2018–2020 from the University of Helsinki (E.M and H.T.), and Bayreuth Humboldt Centre Senior Fellowship 2020 (E. M., A.M., and H.T.). A.M. acknowledges support by the Deutsche Forschungsgemeinschaft (MO2192/6–2) and the European Commission (FET Open NEUROPA, grant agreement 863214).
|EU Grant Number:||
(863214) NEUROPA - Non-invasive dynamic neural control by laser-based technology
© 2022 The Authors. Published by American Chemical Society. This article is published under a CC BY license (Creative Commons Attribution 4.0 International License).