University of Oulu

Nascimento, R.B., Paiva, K.B.S., Risteli, M. et al. Loss of Caveolin-1 Expression in Tumor Cells is Associated with Increased Aggressiveness and Cell Invasion in Oral Squamous Cell Carcinoma. Head and Neck Pathol (2023).

Loss of Caveolin-1 expression in tumor cells is associated with increased aggressiveness and cell invasion in oral squamous cell carcinoma

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Author: Nascimento, Rebeca Barros1; Paiva, Katiúcia Batista Silva2; Risteli, Maija3,4;
Organizations: 1Laboratório de Patologia Oral Cirúrgica, Faculdade de Odontologia, Universidade Federal da Bahia, Rua Araújo Pinho, 62, Canela, Salvador, Bahia 40110-150, Brazil
2Laboratório de Biologia da Matriz Extracelular e Interação Celular, Departamento de Anatomia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil
3Cancer and Translational Medicine Research Unit, Faculty of Medicine, University of Oulu, Oulu, Finland
4Medical Research Center Oulu, Oulu University Hospital, University of Oulu, Oulu, Finland
5Departamento de Ciências Biológicas, Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, Brazil
6Programa de Pós-Graduação em Biofotônica Aplicada a Ciências da Saúde, Universidade Nove de Julho, UNINOVE, São Paulo, Brazil
7Departamento de Cirurgia de Cabeça e Pescoço, Instituto de Câncer Doutor Arnaldo Vieira de Carvalho, São Paulo, Brazil
8Centro de Pesquisa Translacional em Oncologia, Instituto do Câncer do Estado de São Paulo, São Paulo, Brazil
9Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland
10Department of Pathology, HUSLAB, Helsinki University Central Hospital, University of Helsinki, Helsinki, Finland
11Laboratório de Patologia Molecular, Faculdade de Odontologia, Universidade de São Paulo, São Paulo, Brazil
Format: article
Version: accepted version
Access: embargoed
Persistent link:
Language: English
Published: Springer Nature, 2023
Publish Date: 2024-05-26


Background: Changes in Caveolin-1 (CAV-1) expression are related to tumorigenesis. The aim of this study was to evaluate the role of CAV-1 in tumor progression in oral squamous cell carcinoma (SCC) tissue samples and the effect of CAV-1 silencing on two oral tongue SCC (OTSCC) cell lines (SCC-25, from a primary tumor, and HSC-3 from lymph node metastases).

Methods: Mycroarray hybridization, mRNA expression, and immunohistochemistry were performed on OSCC tissue samples and corresponding non-tumoral margin tissues. The effects of CAV-1 silencing (siCAV-1) on cell viability, membrane fluidity, on the expression of epithelial to mesenchymal transition (EMT) markers and on cell migration and invasion capacity of OTSCC cell lines were evaluated.

Results: Microarray showed a greater CAV-1 expression (1.77-fold) in OSCC tumors than in non-tumoral tissues and 2.0-fold more in less aggressive OSCCs. However, significant differences in CAV-1 gene expression were not seen between tumors and non-tumoral margins nor CAV-1 with any clinicopathological parameters. CAV-1 protein was localized both in carcinoma and in spindle cells of the tumor microenvironment (TME), and CAV-1 positive TME cells were associated with smaller/more aggressive tumors, independent of the carcinoma cells’ expression. Silencing of CAV-1 increased cell viability only in SCC-25 cells. It also stimulated the invasion of HSC-3 cells and increased ECAD and BCAT mRNA in these cells; however, the protein levels of the EMT markers were not affected.

Conclusion: Decreased expression of CAV-1 by tumor cells in OSCC and an increase in the TME were associated with increased cell invasiveness and tumor aggressiveness.

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Series: Head and neck pathology
ISSN: 1936-055X
ISSN-E: 1936-0568
ISSN-L: 1936-055X
DOI: 10.1007/s12105-023-01562-w
Type of Publication: A1 Journal article – refereed
Field of Science: 313 Dentistry
3122 Cancers
Funding: This work was supported by the National Council for Scientific and Technological Development (CNPq) [Grant No. 421249/2018–8] and Higher Education Personnel Improvement Coordination (RB Nascimento fellowship—CAPES).
Copyright information: © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. This version of the article has been accepted for publication, after peer review (when applicable) but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: Use of this Accepted Version is subject to the publisher’s Accepted Manuscript terms of use