Otahal, A., De Luna, A., Mobasheri, A., Nehrer, S. (2023). Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering and Osteoarthritis Therapy. In: Stoddart, M.J., Della Bella, E., Armiento, A.R. (eds) Cartilage Tissue Engineering. Methods in Molecular Biology, vol 2598. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2839-3_10
Extracellular vesicle isolation and characterization for applications in cartilage tissue engineering and osteoarthritis therapy
|Author:||Otahal, Alexander1; De Luna, Andrea1; Mobasheri, Ali2,3,4,5,6;|
1Center for Regenerative Medicine, University For Continuing Education, Krems, Austria
2Department of Regenerative Medicine, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania
3Research Unit of Medical Imaging, Physics and Technology, Faculty of Medicine, University of Oulu, Oulu, Finland
4Departments of Orthopedics, Rheumatology and Clinical Immunology, University Medical Center Utrecht, Utrecht, The Netherlands
5Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China
6World Health Organization Collaborating Center for Public Health Aspects of Musculoskeletal Health and Aging, Université de Liège, Liège, Belgium
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe2023070689650
|Publish Date:|| 2024-11-11
Extracellular vesicles (EVs) have the capacity for use in cartilage tissue engineering by stimulating tissue repair and microenvironmental reprogramming. This makes them ideal candidates for treating focal cartilage defects and cartilage degeneration in osteoarthritis (OA). Observational studies have reported beneficial biological effects of EVs, such as inhibition of inflammation, enhanced extracellular matrix deposition, and reduced cartilage degradation. Isolation of EVs derived from different source materials such as conditioned cell culture media or biofluids is essential to attribute observed biological effects to EVs as genuine effectors. This chapter presents a density- and a size-based method as well as a combination of both for isolation of EVs from conditioned cell culture media or biofluids. In addition, three methods for characterization of isolated EVs are suggested based on physical properties, protein profiling, and ultrastructural morphology.
Methods in molecular biology
|Pages:||123 - 140|
Cartilage tissue engineering
|Host publication editor:||
Stoddart, Martin J.
Della Bella, Elena
Armiento, Angela R.
|Type of Publication:||
A3 Book chapter
|Field of Science:||
3121 General medicine, internal medicine and other clinical medicine