University of Oulu

Otahal, A., De Luna, A., Mobasheri, A., Nehrer, S. (2023). Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering and Osteoarthritis Therapy. In: Stoddart, M.J., Della Bella, E., Armiento, A.R. (eds) Cartilage Tissue Engineering. Methods in Molecular Biology, vol 2598. Humana, New York, NY.

Extracellular vesicle isolation and characterization for applications in cartilage tissue engineering and osteoarthritis therapy

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Author: Otahal, Alexander1; De Luna, Andrea1; Mobasheri, Ali2,3,4,5,6;
Organizations: 1Center for Regenerative Medicine, University For Continuing Education, Krems, Austria
2Department of Regenerative Medicine, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania
3Research Unit of Medical Imaging, Physics and Technology, Faculty of Medicine, University of Oulu, Oulu, Finland
4Departments of Orthopedics, Rheumatology and Clinical Immunology, University Medical Center Utrecht, Utrecht, The Netherlands
5Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China
6World Health Organization Collaborating Center for Public Health Aspects of Musculoskeletal Health and Aging, Université de Liège, Liège, Belgium
Format: article
Version: accepted version
Access: embargoed
Persistent link:
Language: English
Published: Humana Press, 2023
Publish Date: 2024-11-11


Extracellular vesicles (EVs) have the capacity for use in cartilage tissue engineering by stimulating tissue repair and microenvironmental reprogramming. This makes them ideal candidates for treating focal cartilage defects and cartilage degeneration in osteoarthritis (OA). Observational studies have reported beneficial biological effects of EVs, such as inhibition of inflammation, enhanced extracellular matrix deposition, and reduced cartilage degradation. Isolation of EVs derived from different source materials such as conditioned cell culture media or biofluids is essential to attribute observed biological effects to EVs as genuine effectors. This chapter presents a density- and a size-based method as well as a combination of both for isolation of EVs from conditioned cell culture media or biofluids. In addition, three methods for characterization of isolated EVs are suggested based on physical properties, protein profiling, and ultrastructural morphology.

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Series: Methods in molecular biology
ISSN: 1064-3745
ISSN-E: 1940-6029
ISSN-L: 1064-3745
ISBN: 978-1-0716-2839-3
ISBN Print: 978-1-0716-2838-6
Issue: 2598
Pages: 123 - 140
DOI: 10.1007/978-1-0716-2839-3_10
Host publication: Cartilage tissue engineering
Host publication editor: Stoddart, Martin J.
Della Bella, Elena
Armiento, Angela R.
Type of Publication: A3 Book chapter
Field of Science: 3121 General medicine, internal medicine and other clinical medicine
Copyright information: © 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. This version of the article has been accepted for publication, after peer review (when applicable) but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: Use of this Accepted Version is subject to the publisher’s Accepted Manuscript terms of use