University of Oulu

Juusti V, Kulpakko J, Cudjoe E, Pimenoff VN, Hänninen P. Biophysical Properties of Bifunctional Phage-Biosensor. Viruses. 2023; 15(2):299.

Biophysical properties of bifunctional phage-biosensor

Saved in:
Author: Juusti, Vilhelmiina1,2; Kulpakko, Janne2; Cudjoe, Elizabeth3;
Organizations: 1Laboratory of Biophysics and Medicity Research Laboratories, Institute of Biomedicine, Faculty of Medicine, University of Turku, Tykistökatu 6A, 20520 Turku, Finland
2Aqsens Health Ltd., Itäinen Pitkäkatu 4B, 20520 Turku, Finland
3Immunology Department, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon P.O. Box LG581, Ghana
4Biobank Borealis of Northern Finland, Faculty of Medicine, University of Oulu, Aapistie 5B, 90220 Oulu, Finland
5Department of Clinical Science, Intervention and Technology, Karolinska Institutet, 14186 Stockholm, Sweden
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 18 MB)
Persistent link:
Language: English
Published: Multidisciplinary Digital Publishing Institute, 2023
Publish Date: 2023-07-12


Biosensor research is a swiftly growing field for developing rapid and precise analytical devices for biomedical, pharmaceutical, and industrial use and beyond. Herein, we propose a phage-based biosensor method to develop a sensitive and specific system for biomedical detection. Our method is based on in vitro selected phages and their interaction with the targeted analytes as well as on optical properties that change according to the concentration of the model analyte. The green fluorescent protein (GFP) was chosen as our model analyte as it has its own well-known optical properties. Brilliant green was used as a reporter component for the sensor. Its presence enables a color intensity (absorbance) change when the analyte is present in the solution. Furthermore, the reporter dye functioned as a quencher for an additional lanthanide label in our assay. It mediated the specific phage-derived interference in the signal measured with the time-resolved luminescence. Most importantly, our results confirmed that the presented bifunctional phage with its liquid crystal properties enabled the measurement of GFP in a concentration-dependent, quantitative manner with a limit of detection of 0.24 µg/mL. In the future, our novel method to develop phage-based biosensors may provide highly sensitive and specific biosensors for biomedical or otherwise-relevant targets.

see all

Series: Viruses
ISSN: 1999-4915
ISSN-E: 1999-4915
ISSN-L: 1999-4915
Volume: 15
Issue: 2
Article number: 299
DOI: 10.3390/v15020299
Type of Publication: A1 Journal article – refereed
Field of Science: 3111 Biomedicine
Funding: This research was funded by Aqsens Health, Itäinen Pitkäkatu 4B, 20520 Turku, Finland.
Copyright information: © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (