University of Oulu

Vuoti, E., Lehenkari, P., Tuukkanen, J. et al. Osteoclastogenesis of human peripheral blood, bone marrow, and cord blood monocytes. Sci Rep 13, 3763 (2023).

Osteoclastogenesis of human peripheral blood, bone marrow, and cord blood monocytes

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Author: Vuoti, Ella1; Lehenkari, Petri1; Tuukkanen, Juha1;
Organizations: 1Institute of Cancer Research and Translational Medicine, Department of Anatomy and Cell Biology, Medical Research Center, University of Oulu, P.O. Box 5000, 90014, Oulu, Finland
2Research Unit of Biomedicine, University of Oulu, Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 2.5 MB)
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Language: English
Published: Springer Nature, 2023
Publish Date: 2023-09-19


Osteoclasts are multinucleated bone resorbing cells that can be differentiated from human monocytes in vitro. There are few studies comparing osteoclastogenesis of different monocyte sources. We compared monocytes from human bone marrow (BM), peripheral blood (PB), and umbilical cord blood (CB) and their osteoclastogenic potential by culturing them with RANKL (20 and 80 ng/ml) and M-CSF (10 ng/ml) for 14 days. We also cultured cells without growth factors, as umbilical cord blood monocytes have been reported to be able to fuse spontaneously into osteoclasts. The data was analysed on d4, d8, d11, and d14. After culture with RANKL and M-CSF, all types of cell cultures developed TRACP -positive multinuclear cells that were able to form resorption pits on human bone slices. Only occasional multinuclear cells and small infrequent resorbed areas could be found in PB and CB-derived cultures without growth factors. BM-derived cells formed greater resorption areas than PB- and CB-derived monocytes. The greatest monocyte population in BM samples were intermediate (CD14⁺⁺CD16⁺) and in PB and CB classical monocytes (76.3% and 54.4%, respectively). In conclusion, our data demonstrates that bone resorbing osteoclasts can be differentiated from BM, PB and CB. However, the osteoclast precursor origin can affect the osteoclast properties and function.

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Series: Scientific reports
ISSN: 2045-2322
ISSN-E: 2045-2322
ISSN-L: 2045-2322
Volume: 13
Issue: 1
Article number: 3763
DOI: 10.1038/s41598-023-30701-0
Type of Publication: A1 Journal article – refereed
Field of Science: 3122 Cancers
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