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Scanning transmission soft X-ray spectromicroscopy of mouse kidney and liver |
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| Author: | Mansikkala, Tuomas1,2; Ohigashi, Takuji3; Salo, Miia H.2,4; |
| Organizations: |
1Nano and Molecular Systems Research Unit, University of Oulu, PO Box 3000, 90014 Oulu, Finland 2Biocenter Oulu, PO Box 5000, 90014 Oulu, Finland 3UVSOR Synchrotron Facility, Institute for Molecular Science, Okazaki 444-8585, Japan
4Research Unit of Clinical Medicine and Medical Research Center, Oulu University Hospital and University of Oulu, 90014 Oulu, Finland
5Research Centre for Integrative Physiology and Pharmacology, and Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, 20014 Turku, Finland 6Stem Cells and Metabolism Research, Program, Faculty of Medicine, University of Helsinki, 00014 Helsinki, Finland 7GM-Unit, Laboratory Animal Center, Helsinki Institute of Life Science, University of Helsinki, 00014 Helsinki, Finland 8Clinic for Children and Adolescents, Oulu University Hospital, 90029 Oulu, Finland |
| Format: | article |
| Version: | published version |
| Access: | open |
| Online Access: | PDF Full Text (PDF, 4.6 MB) |
| Persistent link: | http://urn.fi/urn:nbn:fi-fe20230921134638 |
| Language: | English |
| Published: |
Elsevier,
2023
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| Publish Date: | 2023-09-21 |
| Description: |
AbstractScanning transmission X-ray microscopy (STXM) in the soft X-ray range is well-suited to study ultrastructural features of mammalian soft tissues. Especially at the carbon 1s edge, the imaging contrast varies drastically across the edge due to rapid changes in the X-ray absorption cross-section of functional groups present in the tissue samples enabling label-free soft X-ray spectromicroscopic studies. We present STXM spectromicroscopic imaging of mouse kidney and liver tissues. We especially concentrate on ultrastructural abnormalities in genetically modified Slc17a5 mice. STXM is a promising technique to study storage diseases without chemical alteration due to staining agents, but sample preparation poses a challenge. see all
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| Series: |
Journal of electron spectroscopy and related phenomena |
| ISSN: | 0368-2048 |
| ISSN-E: | 1873-2526 |
| ISSN-L: | 0368-2048 |
| Volume: | 266 |
| Article number: | 147368 |
| DOI: | 10.1016/j.elspec.2023.147368 |
| OADOI: | https://oadoi.org/10.1016/j.elspec.2023.147368 |
| Type of Publication: |
A1 Journal article – refereed |
| Field of Science: |
3121 General medicine, internal medicine and other clinical medicine 114 Physical sciences |
| Subjects: | |
| Funding: |
We acknowledge Biocenter Oulu (University of Oulu) “Emerging projects”-program and Jane and Aatos Erkko Foundation (FinnDisMice consortium: R.H., P.S., and S.K.) for funding. This work was also partially supported by the Academy of Finland (Decision Numbers #331436 (J.U.), #296338 & #326291 (M.P.)), Pediatric Research Foundation, Finland (J.U. and R.H.), and Special State Grants for Health Research, Oulu University Hospital, Finland (J.U.). The research was also partially supported by Magnus Ehrnrooth Foundation. T.M. acknowledges funding from Tauno Tönning Foundation (20220043) and Emil Aaltonen Foundation (220134 N1). |
| Academy of Finland Grant Number: |
331436 296338 |
| Detailed Information: |
331436 (Academy of Finland Funding decision) 296338 (Academy of Finland Funding decision) |
| Copyright information: |
© 2023 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
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https://creativecommons.org/licenses/by/4.0/ |