University of Oulu

Mansikkala, T., Ohigashi, T., Salo, M. H., Hiltunen, A. E., Vuolteenaho, R., Sipilä, P., Kuure, S., Huttula, M., Uusimaa, J., Hinttala, R., Miinalainen, I., Kangas, S., & Patanen, M. (2023). Scanning transmission soft X-ray spectromicroscopy of mouse kidney and liver. Journal of Electron Spectroscopy and Related Phenomena, 266, 147368.

Scanning transmission soft X-ray spectromicroscopy of mouse kidney and liver

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Author: Mansikkala, Tuomas1,2; Ohigashi, Takuji3; Salo, Miia H.2,4;
Organizations: 1Nano and Molecular Systems Research Unit, University of Oulu, PO Box 3000, 90014 Oulu, Finland
2Biocenter Oulu, PO Box 5000, 90014 Oulu, Finland
3UVSOR Synchrotron Facility, Institute for Molecular Science, Okazaki 444-8585, Japan
4Research Unit of Clinical Medicine and Medical Research Center, Oulu University Hospital and University of Oulu, 90014 Oulu, Finland
5Research Centre for Integrative Physiology and Pharmacology, and Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, 20014 Turku, Finland
6Stem Cells and Metabolism Research, Program, Faculty of Medicine, University of Helsinki, 00014 Helsinki, Finland
7GM-Unit, Laboratory Animal Center, Helsinki Institute of Life Science, University of Helsinki, 00014 Helsinki, Finland
8Clinic for Children and Adolescents, Oulu University Hospital, 90029 Oulu, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 4.6 MB)
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Language: English
Published: Elsevier, 2023
Publish Date: 2023-09-21


Scanning transmission X-ray microscopy (STXM) in the soft X-ray range is well-suited to study ultrastructural features of mammalian soft tissues. Especially at the carbon 1s edge, the imaging contrast varies drastically across the edge due to rapid changes in the X-ray absorption cross-section of functional groups present in the tissue samples enabling label-free soft X-ray spectromicroscopic studies. We present STXM spectromicroscopic imaging of mouse kidney and liver tissues. We especially concentrate on ultrastructural abnormalities in genetically modified Slc17a5 mice. STXM is a promising technique to study storage diseases without chemical alteration due to staining agents, but sample preparation poses a challenge.

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Series: Journal of electron spectroscopy and related phenomena
ISSN: 0368-2048
ISSN-E: 1873-2526
ISSN-L: 0368-2048
Volume: 266
Article number: 147368
DOI: 10.1016/j.elspec.2023.147368
Type of Publication: A1 Journal article – refereed
Field of Science: 3121 General medicine, internal medicine and other clinical medicine
114 Physical sciences
Funding: We acknowledge Biocenter Oulu (University of Oulu) “Emerging projects”-program and Jane and Aatos Erkko Foundation (FinnDisMice consortium: R.H., P.S., and S.K.) for funding. This work was also partially supported by the Academy of Finland (Decision Numbers #331436 (J.U.), #296338 & #326291 (M.P.)), Pediatric Research Foundation, Finland (J.U. and R.H.), and Special State Grants for Health Research, Oulu University Hospital, Finland (J.U.). The research was also partially supported by Magnus Ehrnrooth Foundation. T.M. acknowledges funding from Tauno Tönning Foundation (20220043) and Emil Aaltonen Foundation (220134 N1).
Academy of Finland Grant Number: 331436
Detailed Information: 331436 (Academy of Finland Funding decision)
296338 (Academy of Finland Funding decision)
Copyright information: © 2023 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (