Zhao, Y., Hubarevich, A., De Fazio, A. F., Iarossi, M., Huang, J.-A., & De Angelis, F. (2023). Plasmonic Bowl-Shaped Nanopore for Raman Detection of Single DNA Molecules in Flow-Through. Nano Lett. 2023, 23, 4830−4836. American Chemical Society (ACS). https://doi.org/10.1021/acs.nanolett.3c00340
Plasmonic bowl-shaped nanopore for Raman detection of single DNA molecules in flow-through
|Author:||Zhao, Yingqi1,2; Hubarevich, Aliaksandr1; De Fazio, Angela Federica1;|
1Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy
2Faculty of Medicine, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Aapistie 5 A, 90220 Oulu, Finland
|Online Access:||PDF Full Text (PDF, 6.3 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe20230925136924
American Chemical Society,
|Publish Date:|| 2023-09-25
Plasmonic nanopores combined with Raman spectroscopy are emerging as platforms for single-molecule detection and sequencing in label-free mode. Recently, the ability of identifying single DNA bases or amino acids has been demonstrated for molecules adsorbed on plasmonic particles and then delivered into the plasmonic pores. Here, we report on bowl-shaped plasmonic gold nanopores capable of direct Raman detection of single λ-DNA molecules in a flow-through scheme. The bowl shape enables the incident laser to be focused into the nanopore to generate a single intense hot spot with no cut off in pore size. Therefore, we achieved ultrasmall focusing of NIR light in a spot of 3 nm. This enabled us to detect 7 consecutive bases along the DNA chain in flow-through conditions. Furthermore, we found a novel electrofluidic mechanism to manipulate the molecular trajectory within the pore volume so that the molecule is pushed toward the hot spot, thus improving the detection efficiency.
|Pages:||4830 - 4836|
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
318 Medical biotechnology
This work has received funding from the European Union’s Horizon 2020 research and innovation program through the project PROID under grant agreement No. 964363. Jian-An Huang acknowledges the Academy Research Fellow project: TwoPoreProSeq (project number 347652) and DigiHealth project (project number 326291), a strategic profiling project at the University of Oulu that is supported by the Academy of Finland and the University of Oulu.
|Academy of Finland Grant Number:||
347652 (Academy of Finland Funding decision)
© 2023 The Authors. Published by American Chemical Society. This publication is licensed under CC-BY 4.0.