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Development of gut mycobiome in infants and young children : a prospective cohort study |
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| Author: | Turunen, Jenni1,2; Paalanne, Niko1,3; Reunanen, Justus2,4; |
| Organizations: |
1Research Unit of Clinical Medicine, University of Oulu, Oulu, Finland 2Biocenter Oulu, University of Oulu, Oulu, Finland 3Department of Pediatrics and Adolescent Medicine, Oulu University Hospital, Oulu, Finland
4Research Unit of Translational Medicine, University of Oulu, Oulu, Finland
5Ecology and Genetics, Faculty of Science, University of Oulu, Oulu, Finland |
| Format: | article |
| Version: | published version |
| Access: | open |
| Online Access: | PDF Full Text (PDF, 2.1 MB) |
| Persistent link: | http://urn.fi/urn:nbn:fi-fe20230925137189 |
| Language: | English |
| Published: |
Springer Nature,
2023
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| Publish Date: | 2023-09-25 |
| Description: |
AbstractBackground: The composition of the gut fungal microbiome, mycobiome, is likely associated with human health. Yet, the development of gut mycobiome is poorly understood in infants and children. Here we investigate how perinatal events influence the development of gut mycobiome. Methods: In this prospective cohort study of 140 infants, we used ITS gene sequencing of fecal samples from birth to the age of 18 months. We compared gut mycobiome composition according to delivery mode and exposure to intrapartum antibiotics during vaginal delivery. Results: At birth, gut mycobiome were dominated by the genus Candida, at 6-month stool samples by Malassezia and Cystofilobasidium, and the 18-month stool samples by Trichosporon and unidentified fungi. Perinatal factors altered mycobiome. At 18 months, gut mycobiome of infants born vaginally consisted mostly of Trichosporon (32%) and unidentified fungi (31%), while those born via Cesarean section delivery samples had mycobiome dominated by Saccharomyces (50%). At the age of 18 months, those exposed to intrapartum antibiotics had mycobiome dominated by Trichosporon (66%) not seen in those unexposed to antibiotics. Conclusions: Delivery mode and exposure to intrapartum antibiotic prophylaxis were markedly associated with gut mycobiome composition from birth to 18 months of age. Impact: ● The composition of the gut mycobiome is likely associated with human health. Yet, the development of gut mycobiome is poorly understood in infants and children. ● In this prospective cohort study, delivery mode and exposure to intrapartum antibiotic prophylaxis were markedly associated with gut mycobiome composition from birth to 18 months of age. ● The impact of intrapartum antibiotic prophylaxis on fungal microbiome in vaginally born infants, previously shown to influence gut bacteriome composition, may be explained by the interaction between bacteria and fungi. ● Gut mycobiome composition likely deserves further investigation in relation to gut microbiome and health in children. see all
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| Series: |
Pediatric research |
| ISSN: | 0031-3998 |
| ISSN-E: | 1530-0447 |
| ISSN-L: | 0031-3998 |
| Volume: | 94 |
| Issue: | 2 |
| Pages: | 486 - 494 |
| DOI: | 10.1038/s41390-023-02471-y |
| OADOI: | https://oadoi.org/10.1038/s41390-023-02471-y |
| Type of Publication: |
A1 Journal article – refereed |
| Field of Science: |
3123 Gynaecology and paediatrics 1183 Plant biology, microbiology, virology |
| Subjects: | |
| Funding: |
M.V.T. thankfully acknowledges a grant from the Päivikki and Sakari Sohlberg Foundation. T.T. would like to thank the Academy of Finland for a Clinical Research grant in 2018–2022 and a Pediatric Research Foundation grant in 2019–2022. Open Access funding provided by University of Oulu including Oulu University Hospital. |
| Dataset Reference: |
The raw sequences were uploaded in BioProject with the accession number PRJNA831656. |
| Copyright information: |
© The Author(s) 2023. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
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https://creativecommons.org/licenses/by/4.0/ |