Häyrinen MJ, Kiiskilä J, Ranki A, Väkevä L, Barton HJ, Kuusisto MEL, Porvari K, Kuitunen H, Haapasaari K-M, Teppo H-R, et al. The Transcription Factor Twist1 Has a Significant Role in Mycosis Fungoides (MF) Cell Biology: An RNA Sequencing Study of 40 MF Cases. Cancers. 2023; 15(5):1527. https://doi.org/10.3390/cancers15051527
The transcription factor Twist1 has a significant role in mycosis fungoides (MF) cell biology : an RNA sequencing study of 40 MF cases
|Author:||Häyrinen, Marjaana J.1,2; Kiiskilä, Jenni1,2; Ranki, Annamari3;|
1Institute of Clinical Medicine, Faculty of Health Medicine, University of Eastern Finland, 70210 Kuopio, Finland
2Cancer Research and Translational Medicine Research Unit, University of Oulu, 90014 Oulu, Finland
3Department of Skin and Allergic Diseases, University of Helsinki, Helsinki University Central Hospital, P.O. Box 160, 00029 HUS Helsinki, Finland
4Genevia Technologies Oy, 33100 Tampere, Finland
5Department of Haematology, Oulu University Hospital, 90220 Oulu, Finland
6Medical Research Center Oulu, Oulu University Hospital, University of Oulu, 90220 Oulu, Finland
7Cancer Center, Oulu University Hospital, 90220 Oulu, Finland
8Department of Pathology, Oulu University Hospital, 90220 Oulu, Finland
9Cancer Center, Kuopio University Hospital, 70210 Kuopio, Finland
|Online Access:||PDF Full Text (PDF, 5 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe20230927137576
Multidisciplinary Digital Publishing Institute,
|Publish Date:|| 2023-09-27
The purpose of this RNA sequencing study was to investigate the biological mechanism underlying how the transcription factors (TFs) Twist1 and Zeb1 influence the prognosis of mycosis fungoides (MF). We used laser-captured microdissection to dissect malignant T-cells obtained from 40 skin biopsies from 40 MF patients with stage I–IV disease. Immunohistochemistry (IHC) was used to determinate the protein expression levels of Twist1 and Zeb1. Based on RNA sequencing, principal component analysis (PCA), differential expression (DE) analysis, ingenuity pathway analysis (IPA), and hub gene analysis were performed between the high and low Twist1 IHC expression cases. The DNA from 28 samples was used to analyze the TWIST1 promoter methylation level. In the PCA, Twist1 IHC expression seemed to classify cases into different groups. The DE analysis yielded 321 significant genes. In the IPA, 228 significant upstream regulators and 177 significant master regulators/causal networks were identified. In the hub gene analysis, 28 hub genes were found. The methylation level of TWIST1 promoter regions did not correlate with Twist1 protein expression. Zeb1 protein expression did not show any major correlation with global RNA expression in the PCA. Many of the observed genes and pathways associated with high Twist1 expression are known to be involved in immunoregulation, lymphocyte differentiation, and aggressive tumor biology. In conclusion, Twist1 might be an important regulator in the disease progression of MF.
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
This study was funded by Northern Finland Terttu Foundation and Cancer Foundation Finland.
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).