University of Oulu

Neda Alasvand, Aliasghar Behnamghader, Peiman B. Milan, Sara Simorgh, Ali Mobasheri, Masoud Mozafari, Tissue-engineered small-diameter vascular grafts containing novel copper-doped bioactive glass biomaterials to promote angiogenic activity and endothelial regeneration, Materials Today Bio, Volume 20, 2023, 100647, ISSN 2590-0064,

Tissue-engineered small-diameter vascular grafts containing novel copper-doped bioactive glass biomaterials to promote angiogenic activity and endothelial regeneration

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Author: Alasvand, Neda1; Behnamghader, Aliasghar1; Milan, Peiman B.2,3;
Organizations: 1Bioengineering Research Group, Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center (MERC), Tehran, Iran
2Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
3Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
4Research Unit of Health Sciences and Technology, Faculty of Medicine, University of Oulu, Oulu, Finland
5Department of Regenerative Medicine, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania
6Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China
7World Health Organization Collaborating Centre for Public Health Aspects of Musculoskeletal Health and Aging, Liege, Belgium
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 7.8 MB)
Persistent link:
Language: English
Published: Elsevier, 2023
Publish Date: 2023-09-28


Small-diameter vascular grafts frequently fail because of obstruction and infection. Despite the wide range of commercially available vascular grafts, the anatomical uniqueness of defect sites demands patient-specific designs. This study aims to increase the success rate of implantation by fabricating bilayer vascular grafts containing bioactive glasses (BGs) and modifying their composition by removing hemostatic ions to make them blood-compatible and to enhance their antibacterial and angiogenesis properties. The porous vascular graft tubes were 3D printed using polycaprolactone, polyglycerol sebacate, and the modified BGs. The polycaprolactone sheath was then wrapped around the 3D-printed layer using the electrospinning technique to prevent blood leakage. The results demonstrated that the incorporation of modified BGs into the polymeric matrix not only improved the mechanical properties of the vascular graft but also significantly enhanced its antibacterial activity against both gram-negative and gram-positive strains. In addition, no hemolysis or platelet activity was detected after incorporating modified BGs into the vascular grafts. Copper-releasing vascular grafts significantly enhanced endothelial cell proliferation, motility, and VEGF secretion. Additionally, In vivo angiogenesis (CD31 immunofluorescent staining) and gene expression experiments showed that copper-releasing vascular grafts considerably promoted the formation of new blood vessels, low-grade inflammation (decreased expression of IL-1β and TNF-α), and high-level angiogenesis (increased expression of angiogenic growth factors including VEGF, PDGF-BB, and HEBGF). These observations indicate that the use of BGs with suitable compositional modifications in vascular grafts may promote the clinical success of patient-specific vascular prostheses by accelerating tissue regeneration without any coagulation problems.

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Series: Materials today bio
ISSN: 2590-0064
ISSN-E: 2590-0064
ISSN-L: 2590-0064
Volume: 20
Article number: 100647
DOI: 10.1016/j.mtbio.2023.100647
Type of Publication: A1 Journal article – refereed
Field of Science: 216 Materials engineering
213 Electronic, automation and communications engineering, electronics
Funding: Ali Mobasheri is funded by the European Regional Development Fund through the Research Council of Lithuania according to the Programme “Attracting Foreign Researchers for Research Implementation”, Grant No. 01.2.2-LMT-K-718-02-0022 and by the Academy of Finland through the Profi6 336449 grant awarded to the University of Oulu.
Copyright information: © 2023 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (