Lina Lawenius, Carrie Cowardin, Louise Grahnemo, Julia M. Scheffler, Karin Horkeby, Cecilia Engdahl, Jianyao Wu, Liesbeth Vandenput, Antti Koskela, Juha Tuukkanen, Eivind Coward, Kristian Hveem, Arnulf Langhammer, Sanna Abrahamsson, Jeffrey I. Gordon, Klara Sjögren & Claes Ohlsson (2023) Transplantation of gut microbiota from old mice into young healthy mice reduces lean mass but not bone mass, Gut Microbes, 15:1, DOI: 10.1080/19490976.2023.2236755
Transplantation of gut microbiota from old mice into young healthy mice reduces lean mass but not bone mass
|Author:||Lawenius, Lina1; Cowardin, Carrie2; Grahnemo, Louise1;|
1Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Osteoporosis Centre, Centre for Bone and Arthritis Research at the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
2The Edison Family Center for Genome Sciences and Systems Biology, Washington University in St. Louis, St. Louis, MO, USA
3Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Osteoporosis Centre, Centre for Bone and Arthritis Research at the Sahlgrenska Academy, Sahlgrenska Academy University of Gothenburg, Gothenburg, Sweden
4Department of Anatomy and Cell Biology, Faculty of Medicine, Institute of Cancer Research and Translational Medicine, University of Oulu, Oulu, Finland
5K.G. Jebsen Center for Genetic Epidemiology, Department of Public Health and Nursing, NTNU, Norwegian University of Science and Technology, Trondheim, Norway
6HUNT Research Centre, Department of Public Health and Nursing, Norwegian University of Science and Technology, Levanger, Norway
7Bioinformatics and Data Centre, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
8Department of Drug Treatment, Region Västra Götaland, Sahlgrenska University Hospital, Gothenburg, Sweden
|Online Access:||PDF Full Text (PDF, 8.6 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe20230929137788
|Publish Date:|| 2023-09-29
Aging is associated with low bone and lean mass as well as alterations in the gut microbiota (GM). In this study, we determined whether the reduced bone mass and relative lean mass observed in old mice could be transferred to healthy young mice by GM transplantation (GMT). GM from old (21-month-old) and young adult (5-month-old) donors was used to colonize germ-free (GF) mice in three separate studies involving still growing 5- or 11-week-old recipients and 17-week-old recipients with minimal bone growth. The GM of the recipient mice was similar to that of the donors, demonstrating successful GMT. GM from old mice did not have statistically significant effects on bone mass or bone strength, but significantly reduced the lean mass percentage of still growing recipient mice when compared with recipients of GM from young adult mice. The levels of propionate in the cecum of mice receiving old donor GM were significantly lower than those in mice receiving young adult donor GM. Bacteroides ovatus was enriched in the microbiota of recipient mice harboring GM from young adult donors. The presence of B. ovatus was not only significantly associated with high lean mass percentage in mice, but also with lean mass adjusted for fat mass in the large human HUNT cohort. In conclusion, GM from old mice reduces lean mass percentage but not bone mass in young, healthy, still growing recipient mice. Future studies are warranted to determine whether GM from young mice improves the musculoskeletal phenotype of frail elderly recipient mice.
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
This study was supported by the Swedish Research Council (grant numbers 2018-02589 and 2021-01739) and by grants from the Swedish state under the agreement between the Swedish government and the county councils (ALF-agreement:238261), the Lundberg Foundation, and the Knut and Alice Wallenberg Foundation (Wallenberg Clinical Scholars). CC was supported by the NIH T32 AI007172.
© 2023 The Author(s). Published with license by Taylor & Francis Group, LLC. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.