Karjula, T., Niskakangas, A., Mustonen, O. et al. Tertiary lymphoid structures in pulmonary metastases of microsatellite stable colorectal cancer. Virchows Arch 483, 21–32 (2023). https://doi.org/10.1007/s00428-023-03577-8
Tertiary lymphoid structures in pulmonary metastases of microsatellite stable colorectal cancer
|Author:||Karjula, Topias1; Niskakangas, Anne1; Mustonen, Olli1;|
1Translational Medicine Research Unit, Medical Research Center Oulu, Oulu University Hospital and University of Oulu, Aapistie 5a, 90220 Oulu, Finland
2Department of Biological and Environmental Science, University of Jyväskylä, 40014 Jyväskylä, Finland
3Department of Education and Research, Central Finland Health Care District, 40620 Jyväskylä, Finland
4Department of Pathology, Central Finland Health Care District, 40620 Jyväskylä, Finland
5Faculty of Sport and Health Sciences, University of Jyväskylä, 40014 Jyväskylä, Finland
6Faculty of Medicine and Health Technology, Tampere University and TAYS Cancer Center, Tampere University Hospital, 33520 Tampere, Finland
7Department of Gastrointestinal Surgery, Helsinki University Central Hospital, University of Helsinki, 00290 Helsinki, Finland
8Applied Tumor Genomics, Research Program Unit, University of Helsinki, 00290 Helsinki, Finland
9Department of Gastroenterology and Alimentary Tract Surgery, Tampere University Hospital, 33520 Tampere, Finland
10Central Hospital of Central Finland, 40014 Jyväskylä, Finland
11Department of Cardiothoracic Surgery, Oulu University Hospital, Oulu, Finland
|Online Access:||PDF Full Text (PDF, 1.8 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe20230929137827
|Publish Date:|| 2023-09-29
Tertiary lymphoid structures (TLSs) are ectopic lymphoid aggregates located at sites of chronic inflammation and recognized as prognosticators in several cancers. We aimed to analyse the prognostic effect of TLSs in colorectal cancer (CRC) pulmonary metastases and primary tumours, with a comparison to the CD3+ and CD8+ cell density-based immune cell score (ICS). For TLS density and TLS maximum diameter analysis, 67 pulmonary metastases and 63 primary tumours were stained with haematoxylin and eosin. For ICS scoring and analysis, CD3 and CD8 immunohistochemistry was performed. Excellent interobserver agreement was achieved in all TLS measurements. Of all patients, 36 patients had low TLS density (< 0.222 follicles/mm) and 31 patients had high TLS density (≥ 0.222 follicles/mm) in the first resected pulmonary metastases. TLS density (adjusted HR 0.91, 0.48–1.73) or maximum diameter (adjusted HR 0.78, 0.40–1.51) did not have prognostic value in pulmonary metastases. In primary tumours, higher TLS density (adjusted HR 0.39, 0.18–0.87) and maximum diameter (adjusted HR 0.28, 0.11–0.73) were associated with lower mortality. In the pulmonary metastases, ICS had superior prognostic value to TLSs; however, TLSs and ICS were significantly associated. In conclusion, TLSs in CRC pulmonary metastases had no prognostic value but correlated with the ICS. TLSs in primary tumours associated with favourable prognosis.
Virchows Archiv. European journal of pathology
|Pages:||21 - 32|
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
Open Access funding provided by University of Oulu including Oulu University Hospital. This study was funded by Instrumentarium Science Foundation (OH), Mary and Georg C. Ehrnrooth Foundation (OH) and Finnish State Research Funding (OH, J-P.M), Cancer Foundation Finland (J.P.V), J&A Erkko Foundation (J-P.M, TTS).
Data is available from the corresponding author upon reasonable request. Data sharing will require an additional ethical board statement.
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