University of Oulu

Piispanen, J., Bergmann, U., Karhu, J. et al. Variation of compounds in leaves of susceptible and resistant alternate hosts of Cronartium pini and C. ribicola. Eur J Plant Pathol 165, 677–692 (2023).

Variation of compounds in leaves of susceptible and resistant alternate hosts of Cronartium pini and C. ribicola

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Author: Piispanen, Juha1; Bergmann, Ulrich2; Karhu, Jouni1;
Organizations: 1Natural Resources Institute Finland, 90570 Oulu, Finland
2Biocenter, University of Oulu, 90014 Oulu, Finland
3Botanical Gardens, University of Oulu, Oulu 90014, Finland
Format: article
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 0.6 MB)
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Language: English
Published: Springer Nature, 2023
Publish Date: 2023-10-12


Leaf compounds may contribute to plant defense against Cronartium rusts. Secondary compounds are either natural or induced in leaves. We studied the variation of compounds in leaves of six alternate hosts of Cronartium pini and two of C. ribicola that represented either susceptible or resistant species to these rusts. Extracts from the plant leaves were analyzed using LC-MSMS (liquid chromatography tandem mass spectrometry) and compounds were compared between susceptible and resistant species of the same plant genera to identify significant differences between resistant and susceptible species. Also, LC–MS (liquid chromatography mass spectrometry) with external calibration was used to quantify 12 candidate compounds known from the literature. Among these compounds, the most abundant significant ones in C. pini -resistant Melampyrum pratense were chlorogenic acid and quercitrin, in Veronica chamaedrys ferulic acid, quercitrin and luteolin and in Impatiens glandulifera quercitrin, ferulic acid, kaempferol, rutin and hyperoside. In C. ribicola -resistant Ribes rubrum the most abundant significant compounds were caffeic acid, p-coumaric acid and quercitrin. Among all extracted leaf compounds, concentrations of three compounds were over 1000 times greater in rust-resistant M. pratense, three compounds in V. chamaedrys, eight compounds in I. glandulifera, and one compound in R. rubrum than in rust-susceptible species. Among the compounds, the most promising possibly linked to rust resistance were chlorogenic acid and quercitrin.

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Series: European journal of plant pathology
ISSN: 0929-1873
ISSN-E: 1573-8469
ISSN-L: 0929-1873
Volume: 165
Issue: 4
Pages: 677 - 692
DOI: 10.1007/s10658-022-02636-1
Type of Publication: A1 Journal article – refereed
Field of Science: 1183 Plant biology, microbiology, virology
Funding: The study was financed by the Finnish Academy of Science (grant no. 332811). Open access funding provided by Natural Resources Institute Finland (LUKE).
Dataset Reference: The data that support the findings of this study are available from the corresponding author upon reasonable request.
Copyright information: © The Author(s) 2023. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit