Naakka, E., Wahbi, W., Tiikkaja, R. et al. Novel human lymph node-derived matrix supports the adhesion of metastatic oral carcinoma cells. BMC Cancer 23, 750 (2023). https://doi.org/10.1186/s12885-023-11275-6
Novel human lymph node-derived matrix supports the adhesion of metastatic oral carcinoma cells
|Author:||Naakka, Erika1,2; Wahbi, Wafa1,2; Tiikkaja, Riia3,4;|
1Department of Oral and Maxillofacial Diseases, Clinicum, Faculty of Medicine, University of Helsinki, Helsinki, Finland
2Translational Immunology Research Program (TRIMM), University of Helsinki, Helsinki, Finland
3Research Unit of Population Health, Faculty of Medicine, University of Oulu, Oulu, Finland
4Medical Research Center, Oulu University Hospital, University of Oulu, Oulu, Finland
5Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland
6Department of Otorhinolaryngology, Head and Neck Surgery, Oulu University Hospital, Oulu, Finland
7Department of Pathology, Oulu University Hospital, Oulu, Finland
8Department of Obstetrics and Gynecology, Oulu University Hospital, Oulu, Finland
9Institute of Dentistry, School of Medicine, University of Eastern Finland, Kuopio Campus, Kuopio, Finland
10Meilahti Clinical Proteomics Core Facility, Faculty of Medicine, HiLIFE, University of Helsinki, Helsinki, Finland
11Research Unit of Biomedicine, Faculty of Medicine, University of Oulu, Oulu, Finland
12Biocenter Oulu, Oulu, Finland
13Department of Pathology, HUSLAB, Helsinki University Central Hospital, University of Helsinki, Helsinki, Finland
|Online Access:||PDF Full Text (PDF, 2.5 MB)|
|Persistent link:|| http://urn.fi/urn:nbn:fi-fe20231023140855
|Publish Date:|| 2023-10-23
Background: 3D culture is increasingly used in cancer research, as it allows the growth of cells in an environment that mimics in vivo conditions. Metastases are the primary cause of morbidity and mortality in cancer patients, and solid tumour metastases are mostly located in lymph nodes. Currently, there are no techniques that model the pre-metastatic lymph node microenvironment in vitro. In this study, we prepared a novel extracellular matrix, Lymphogel, which is derived from lymph nodes, mimicking the tumour microenvironment (TME) of metastatic carcinoma cells. We tested the suitability of the new matrix in various functional experiments and compared the results with those obtained using existing matrices.
Methods: We used both commercial and patient-derived primary and metastatic oral tongue squamous cell carcinoma (OTSCC) cell lines. We characterized the functional differences of these cells using three different matrices (human uterine leiomyoma-derived Myogel, human pre-metastatic neck lymph node-derived Lymphogel (h-LG), porcine normal neck lymph node-derived Lymphogel (p-LG) in proliferation, adhesion, migration and invasion assays. We also performed proteomic analyses to compare the different matrices in relation to their functional properties.
Results: OTSCC cells exhibited different adhesion and invasion patterns depending on the matrix. Metastatic cell lines showed improved ability to adhere to h-LG, but the effects of the matrices on cell invasion fluctuated non-significantly between the cell lines. Proteomic analyses showed that the protein composition between matrices was highly variable; Myogel contained 618, p-LG 1823 and h-LG 1520 different proteins. The comparison of all three matrices revealed only 120 common proteins. Analysis of cellular pathways and processes associated with proteomes of each matrix revealed similarities of Myogel with h-LG but less with p-LG. Similarly, p-LG contained the least adhesion-related proteins compared with Myogel and h-LG. The highest number of unique adhesion-related proteins was present in h-LG.
Conclusions: We demonstrated that human pre-metastatic neck lymph node-derived matrix is suitable for studying metastatic OTSCC cells. As a whole-protein extract, h-LG provides new opportunities for in vitro carcinoma cell culture experiments.
|Type of Publication:||
A1 Journal article – refereed
|Field of Science:||
Open Access funding provided by University of Oulu including Oulu University Hospital. The work was supported by research grants from the Jane and Aatos Erkko Foundation, the Sigrid Juselius Foundation, the Cancer Foundation Finland, the Academy of Finland (#308363, PÅ), the Finnish Dental Society Apollonia, and the Doctoral Programme in Clinical Research, and by research funds from the Medical Faculty of the University of Oulu and from the Oulu and Helsinki University Hospital special state support for research.
|Academy of Finland Grant Number:||
308363 (Academy of Finland Funding decision)
© The Author(s) 2023. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.