University of Oulu

Mass spectrometric characterization of urinary fibrinogen-derived peptides in prostate cancer and renal cell carcinoma

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Author: Mesihää, Markus1
Organizations: 1University of Oulu, Faculty of Biochemistry and Molecular Medicine, Biochemistry
Format: ebook
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 1.7 MB)
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Language: English
Published: Oulu : M. Mesihää, 2015
Publish Date: 2015-12-11
Thesis type: Master's thesis
Tutor: Stenman, Ulf-Håkan
Reviewer: Ohlmeier, Steffen
Karppinen, Sanna-Maria
In previous studies we have found that urinary fibrinogen-derived peptides are potential tumor markers for renal cell carcinoma. These peptides occur at low concentrations in urine. Identification of a low-abundant tumor marker requires optimal sample preparation and a highly sensitive analyzer. In this work different chromatographic and mass spectrometric methods were compared and evaluated for tumor marker discovery. We used urine samples from patients with renal cell carcinoma and prostate cancer. Our main targets were peptides derived from fibrinogen beta with unknown sequence that are produced by differential proteolysis. With our optimized workflow we discovered 26 fibrinogen beta derived peptides that have not been identified in urine previously.
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Copyright information: © Markus Mesihää, 2015. This publication is copyrighted. You may download, display and print it for your own personal use. Commercial use is prohibited.