University of Oulu

Transcription factor GATA6 in ductal metaplasia of hepatocytes

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Author: Liljeström, Emmi1
Organizations: 1University of Oulu, Faculty of Science, Biology
Format: ebook
Version: published version
Access: open
Online Access: PDF Full Text (PDF, 0.9 MB)
Pages: 23
Persistent link: http://urn.fi/URN:NBN:fi:oulu-201902051147
Language: English
Published: Oulu : E. Liljeström, 2019
Publish Date: 2019-02-13
Thesis type: Bachelor's thesis
Description:

Abstract

GATA6 is evolutionary highly conserved transcription factor and a newish indicator and possible causer of ductal metaplasia in biliary atresia (BA). BA is a rare neonatal cholestatic liver disease caused by fibroinflammatory obstruction of bile ducts. BA causes proliferation of bile ducts which is also called ductal metaplasia. In ductal metaplasia hepatocytes phenotype starts resembling more cholangiocytes. When bile gathers inside the liver it (liver) starts to create new bile ducts. This reaction is called ductal reaction what is a consequence of ductal metaplasia.

In this thesis work we, study the role of GATA6 in ductal metaplasia. We have two different kinds of mice: control mouse (ctrl) and knock out mouse (cKO). Both mice were operated with bile duct ligation (BDL) to resemble the BA-like condition. Our control (Ctrl) mouse only went over BDL and cKO mouse’s GATA6 gene was silenced in addition of the BDL.

Missing of GATA6 has shown histology changes in liver tissue — there is no clear bile duct structure like in normal BA-like tissue. Post-mortem tells also a bad condition of the liver of cKO mouse. Similarly, gene expression studies supports hypothesis that ductal reaction hardly ever happens when GATA6 is missing.

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Copyright information: © Emmi Liljeström, 2019. This publication is copyrighted. You may download, display and print it for your own personal use. Commercial use is prohibited.