Shape-dependent interaction of gold nanoparticles with cultured cells at laser exposure
Bibikova, O.; Singh, P.; Popov, A.; Akchurin, G.; Skaptsov, A.; Skovorodkin, I.; Khanadeev, V.; Mikhalevich, D.; Kinnunen, M.; Akchurin, G.; Bogatyrev, V.; Khlebtsov, N.; Vainio, S.; Meglinski, I.; Tuchin, V. (2017-03-20)
Bibikova, O., Singh, P., Popov, A., Akchurin, G., Skaptsov, A., Skovorodkin, I., … Tuchin, V. (2017). Shape-dependent interaction of gold nanoparticles with cultured cells at laser exposure. Laser Physics Letters, 14(5), 55901. https://doi.org/10.1088/1612-202x/aa63ae
© 2017 Astro Ltd. This is an author-created, un-copyedited version of an article published in Laser Physics Letters. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at https://doi.org/10.1088/1612-202X/aa63ae.
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https://urn.fi/URN:NBN:fi-fe2019050213997
Tiivistelmä
Abstract
Laser optoporation of cells by local heating of plasmonic gold nanoparticles (GNPs) was proven as a favorable delivery method of molecules into cells. The optoporation efficiency depends on the laser beam intensity and GNP properties. Here, we evaluate the membrane optoporation in vitro in terms of fluorescent dye permeability under treatment of a multi-pulsed nanosecond 1064 nm laser with a sharply-focused beam. Anisotropic GNPs, such as nanorods and nanostars, were fabricated to achieve the optimal GNP-cell interaction. Nanostars demonstrated highest optoporation efficacy with more than 80% of permeabilized cells within the illuminated area. By contrast to common laser techniques, the laser beam scanning method results in cell optoporation within a controllable programmed in advance irradiated area.
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