A fully online sensor-equipped, disposable multiphase microbioreactor as a screening platform for biotechnological applications
Maldonado, Susanna Lladó; Panjan, Peter; Sun, Shiwen; Rasch, Detlev; Sesay, Adama M.; Mayr, Torsten; Krull, Rainer (2018-09-10)
Lladó Maldonado, S, Panjan, P, Sun, S, et al. A fully online sensor‐equipped, disposable multiphase microbioreactor as a screening platform for biotechnological applications. Biotechnology and Bioengineering. 2019; 116: 65– 75. Lladó Maldonado, S, Panjan, P, Sun, S, et al. A fully online sensor‐equipped, disposable multiphase microbioreactor as a screening platform for biotechnological applications. Biotechnology and Bioengineering. 2019; 116: 65– 75. https://doi.org/10.1002/bit.26831
© 2018 Wiley Periodicals, Inc. This is the peer reviewed version of the following article: Lladó Maldonado, S, Panjan, P, Sun, S, et al. A fully online sensor‐equipped, disposable multiphase microbioreactor as a screening platform for biotechnological applications. Biotechnology and Bioengineering. 2019; 116: 65– 75, which has been published in final form at Lladó Maldonado, S, Panjan, P, Sun, S, et al. A fully online sensor‐equipped, disposable multiphase microbioreactor as a screening platform for biotechnological applications. Biotechnology and Bioengineering. 2019; 116: 65– 75. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
https://rightsstatements.org/vocab/InC/1.0/
https://urn.fi/URN:NBN:fi-fe2019093030475
Tiivistelmä
Abstract
A new disposable, multiphase, microbioreactor (MBR; with a working volume of 550 μl) equipped with online sensors is presented for biotechnological screening research purposes owing to its high‐throughput potential. Its design and fabrication, online sensor integration, and operation are described. During aerobic cultivation, sufficient oxygen supply is the most important factor that influences growth and product formation. The MBR is a microbubble column bioreactor (μBC), and the oxygen supply was realized by active pneumatic bubble aeration, ensuring sufficient volumetric liquid‐phase mass transfer (kLa) and proper homogenization of the cultivation broth. The μBC was equipped with miniaturized sensors for the pH, dissolved oxygen, optical density and glucose concentration that allowed real‐time online monitoring of these process variables during cultivation. The challenge addressed here was the integration of sensors in the limited available space. The MBR was shown to be a suitable screening platform for the cultivation of biological systems. Batch cultivations of Saccharomyces cerevisiae were performed to observe the variation in the process variables over time and to show the robustness and operability of all the online sensors in the MBR.
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