Non-canonical translation initiation in yeast generates a cryptic pool of mitochondrial proteins
Monteuuis, Geoffray; Miścicka, Anna; Świrski, Michał; Zenad, Lounis; Niemitalo, Olli; Wrobel, Lidia; Alam, Jahangir; Chacinska, Agnieszka; Kastaniotis, Alexander J.; Kufel, Joanna (2019-04-24)
Geoffray Monteuuis, Anna Miścicka, Michał Świrski, Lounis Zenad, Olli Niemitalo, Lidia Wrobel, Jahangir Alam, Agnieszka Chacinska, Alexander J Kastaniotis, Joanna Kufel, Non-canonical translation initiation in yeast generates a cryptic pool of mitochondrial proteins, Nucleic Acids Research, Volume 47, Issue 11, 20 June 2019, Pages 5777–5791, https://doi.org/10.1093/nar/gkz301
© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
https://creativecommons.org/licenses/by-nc/4.0/
https://urn.fi/URN:NBN:fi-fe2019101032179
Tiivistelmä
Abstract
Utilization of non-AUG alternative translation start sites is most common in bacteria and viruses, but it has been also reported in other organisms. This phenomenon increases proteome complexity by allowing expression of multiple protein isoforms from a single gene. In Saccharomyces cerevisiae, a few described cases concern proteins that are translated from upstream near-cognate start codons as N-terminally extended variants that localize to mitochondria. Using bioinformatics tools, we provide compelling evidence that in yeast the potential for producing alternative protein isoforms by non-AUG translation initiation is much more prevalent than previously anticipated and may apply to as many as a few thousand proteins. Several hundreds of candidates are predicted to gain a mitochondrial targeting signal (MTS), generating an unrecognized pool of mitochondrial proteins. We confirmed mitochondrial localization of a subset of proteins previously not identified as mitochondrial, whose standard forms do not carry an MTS. Our data highlight the potential of non-canonical translation initiation in expanding the capacity of the mitochondrial proteome and possibly also other cellular features.
Kokoelmat
- Avoin saatavuus [31657]