Characterization of immune response against <em>Mycobacterium marinum</em> infection in the main hematopoietic organ of adult zebrafish (<em>Danio rerio</em>)
Harjula, Sanna-Kaisa E.; Saralahti, Anni K.; Ojanen, Markus J.T.; Rantapero, Tommi; Uusi-Mäkelä, Meri I.E.; Nykter, Matti; Lohi, Olli; Parikka, Mataleena; Rämet, Mika (2019-10-15)
Sanna-Kaisa E. Harjula, Anni K. Saralahti, Markus J.T. Ojanen, Tommi Rantapero, Meri I.E. Uusi-Mäkelä, Matti Nykter, Olli Lohi, Mataleena Parikka, Mika Rämet, Characterization of immune response against Mycobacterium marinum infection in the main hematopoietic organ of adult zebrafish (Danio rerio), Developmental & Comparative Immunology, Volume 103, 2020, 103523, ISSN 0145-305X, https://doi.org/10.1016/j.dci.2019.103523
© 2019 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).
https://creativecommons.org/licenses/by-nc-nd/4.0/
https://urn.fi/URN:NBN:fi-fe2020040110060
Tiivistelmä
Abstract
Tuberculosis remains a major global health challenge. To gain information about genes important for defense against tuberculosis, we used a well-established tuberculosis model; Mycobacterium marinum infection in adult zebrafish. To characterize the immunological response to mycobacterial infection at 14 days post infection, we performed a whole-genome level transcriptome analysis using cells from kidney, the main hematopoietic organ of adult zebrafish. Among the upregulated genes, those associated with immune signaling and regulation formed the largest category, whereas the largest group of downregulated genes had a metabolic role. We also performed a forward genetic screen in adult zebrafish and identified a fish line with severely impaired survival during chronic mycobacterial infection. Based on transcriptome analysis, these fish have decreased expression of several immunological genes. Taken together, these results give new information about the genes involved in the defense against mycobacterial infection in zebrafish.
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