Extracellular vesicles derived from cancer-associated fibroblasts induce the migration and invasion of oral squamous cell carcinoma
Dourado, Mauricio Rocha; Korvala, Johanna; Åström, Pirjo; De Oliveira, Carine Ervolino; Cervigne, Nilva K.; Mofatto, Luciana Souto; Bastos, Debora Campanella; Pereira Messetti, Ana Camila; Graner, Edgard; Paes Leme, Adriana Franco; Coletta, Ricardo D.; Salo, Tuula (2019-02-13)
Mauricio Rocha Dourado, Johanna Korvala, Pirjo Åström, Carine Ervolino De Oliveira, Nilva K. Cervigne, Luciana Souto Mofatto, Debora Campanella Bastos, Ana Camila Pereira Messetti, Edgard Graner, Adriana Franco Paes Leme, Ricardo D. Coletta & Tuula Salo (2019) Extracellular vesicles derived from cancer-associated fibroblasts induce the migration and invasion of oral squamous cell carcinoma, Journal of Extracellular Vesicles, 8:1, DOI: 10.1080/20013078.2019.1578525
© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles.This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), whichpermits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
https://creativecommons.org/licenses/by-nc/4.0/
https://urn.fi/URN:NBN:fi-fe2020040810763
Tiivistelmä
Abstract
As one of the most abundant constituents of the tumour microenvironment (TME), cancer-associated fibroblasts (CAF) display critical roles during tumour progression and metastasis. Multiple classes of molecules including growth factors, cytokines, proteases and extracellular matrix proteins, are produced by CAF to act as mediators of the stroma-tumour interactions. One of the main channels for this communication is associated with extracellular vesicles (EV), which are secreted particles loaded with protein and genetic information. In this study, we evaluated the effects of EV derived from CAF primary human cell lines (n = 5) on proliferation, survival, migration, and invasion of oral squamous cell carcinoma (OSCC) cells. As controls, EV from human primary-established normal oral fibroblasts (NOF, n = 5) were used. Our in vitro assays showed that CAF-EV significantly induces migration and invasion of OSCC cells and promote a disseminated pattern of HSC-3 cell invasion in the 3D organotypic assay. Furthermore, gene expression analysis of EV-treated cancer cells revealed changes in the pathways associated with tumour metabolism and up-regulation of tumour invasion genes. Our findings suggest a significant role of CAF-EV in promoting the migration and invasion of OSCC cells, which are related to the activation of cancer-related pathways.
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