Tonsil mycobiome in PFAPA (periodic fever, aphthous stomatitis, pharyngitis, adenitis) syndrome : a case-control study
Tejesvi, Mysore V.; Tapiainen, Terhi; Vänni, Petri; Uhari, Matti; Suokas, Marko; Lantto, Ulla; Koivunen, Petri; Renko, Marjo (2021-01-27)
Tejesvi MV, Tapiainen T, Vänni P, Uhari M, Suokas M, Lantto U, Koivunen P and Renko M (2021) Tonsil Mycobiome in PFAPA (Periodic Fever, Aphthous Stomatitis, Pharyngitis, Adenitis) Syndrome: A Case-Control Study. Front. Cell. Infect. Microbiol. 10:616814. doi: 10.3389/fcimb.2020.616814
© 2021 Tejesvi, Tapiainen, Vänni, Uhari, Suokas, Lantto, Koivunen and Renko. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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https://urn.fi/URN:NBN:fi-fe2021041510578
Tiivistelmä
Abstract
Periodic fever, aphthous stomatitis, pharyngitis and adenitis syndrome (PFAPA) is the most common periodic fever syndrome in children with unknown etiology, effectively treated with tonsillectomy. Earlier we have shown that tonsil microbiome is different in patients with PFAPA as compared to that in controls. Recently, fungal microbiome, mycobiome, has been linked to the pathogenesis of inflammatory diseases. We now investigated the role of mycobiome of tonsils in PFAPA. Random forest classification, a machine learning approach, was used for the analysis of mycobiome data. We examined tonsils from 30 children with PFAPA and 22 control children undergoing tonsillectomy for non-infectious reasons. We identified 103 amplicon sequence variants, mainly from two fungal phyla, Ascomycota and Basidiomycota. The mean relative abundance of Candida albicans in the tonsil mycobiome was 11% (95% CI: 19 to 27%) in cases and 3.4 % (95% CI: -0.8% to 8%) in controls, p =0.104. Mycobiome data showed no statistical difference in differentiating between PFAPA cases and controls compared to a random chance classifier (area under the curve (AUC) = 0.47, SD = 0.05, p = 0.809). In conclusion, in this controlled study, tonsillar mycobiome in children with PFAPA syndrome did not differ from that of the controls.
Kokoelmat
- Avoin saatavuus [32009]