Umbilical cord blood DNA methylation in children who later develop type 1 diabetes
Laajala, Essi; Kalim, Ubaid Ullah; Grönroos, Toni; Rasool, Omid; Halla-aho, Viivi; Konki, Mikko; Kattelus, Roosa; Mykkänen, Juha; Nurmio, Mirja; Vähä-Mäkilä, Mari; Kallionpää, Henna; Lietzén, Niina; Ghimire, Bishwa R.; Laiho, Asta; Hyöty, Heikki; Elo, Laura L.; Ilonen, Jorma; Knip, Mikael; Lund, Riikka J.; Orešič, Matej; Veijola, Riitta; Lähdesmäki, Harri; Toppari, Jorma; Lahesmaa, Riitta (2022-06-18)
Laajala, E., Kalim, U.U., Grönroos, T. et al. Umbilical cord blood DNA methylation in children who later develop type 1 diabetes. Diabetologia 65, 1534–1540 (2022). https://doi.org/10.1007/s00125-022-05726-1
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https://urn.fi/URN:NBN:fi-fe2022122072816
Tiivistelmä
Abstract
Aims/hypothesis: Distinct DNA methylation patterns have recently been observed to precede type 1 diabetes in whole blood collected from young children. Our aim was to determine whether perinatal DNA methylation is associated with later progression to type 1 diabetes.
Methods: Reduced representation bisulphite sequencing (RRBS) analysis was performed on umbilical cord blood samples collected within the Finnish Type 1 Diabetes Prediction and Prevention (DIPP) Study. Children later diagnosed with type 1 diabetes and/or who tested positive for multiple islet autoantibodies (n = 43) were compared with control individuals (n = 79) who remained autoantibody-negative throughout the DIPP follow-up until 15 years of age. Potential confounding factors related to the pregnancy and the mother were included in the analysis.
Results: No differences in the umbilical cord blood methylation patterns were observed between the cases and controls at a false discovery rate <0.05.
Conclusions/interpretation: Based on our results, differences between children who progress to type 1 diabetes and those who remain healthy throughout childhood are not yet present in the perinatal DNA methylome. However, we cannot exclude the possibility that such differences would be found in a larger dataset.
Kokoelmat
- Avoin saatavuus [31978]