SPICY : a method for single scan rotating frame relaxometry
Tolkkinen, Katja; Mailhiot, Sarah E.; Selent, Anne; Mankinen, Otto; Henschel, Henning; Nieminen, Miika T.; Hanni, Matti; Kantola, Anu M.; Liimatainen, Timo; Telkki, Ville-Veikko (2023-04-13)
Phys. Chem. Chem. Phys., 2023,25, 13164-13169
This journal is © the Owner Societies 2023. Open Access Article. Published on 13 April 2023. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
https://creativecommons.org/licenses/by/3.0/
https://urn.fi/URN:NBN:fi-fe2023051243675
Tiivistelmä
Abstract
T1ρ is an NMR relaxation mode that is sensitive to low frequency molecular motions, making it an especially valuable tool in biomolecular research. Here, we introduce a new method, SPICY, for measuring T1ρ relaxation times. In contrast to conventional T1ρ experiments, in which the sequence is repeated many times to determine the T1ρ time, the SPICY sequence allows determination of T1ρ within a single scan, shortening the experiment time remarkably. We demonstrate the method using ¹H T1ρ relaxation dispersion experiments. Additionally, we combine the sequence with spatial encoding to produce 1D images in a single scan. We show that T1ρ relaxation times obtained using the single scan approach are in good agreement with those obtained using the traditional experiments.
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